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Dec 14, 2014 (publication date) through Oct 25, 2025
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World Journal of Gastroenterology
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1007-9327
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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Original Article
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Dec 14, 2014; 20(46): 17376-17387
Published online Dec 14, 2014. doi: 10.3748/wjg.v20.i46.17376
Table 1 Primer sequences used in quantitative polymerase chain reaction analysis
Target geneForwardReverse
GAPDHACCCACTCCTCCACCTTTGACATACCAGGAAATGAGCTTGACAA
PEA3AGGAGACGTGGCTCGCTGAAACCTAGCTTTCCACAGCCCC
FSP1TGATCCTGACTGCTGTCATGGTACTCTTGGAAGTCCACCTCG
TWISTGACCTCGGGGCCATCCACACCCGCCGCCGCCGCCACAGC
VIMENTINGCGTGACGTACGTCAGCAATATGAGTTCCAGGGACTCATTGGTTCCTT
SLUGACGCCTCCAAAAAGCCAAACTACACTTCAGGGCGCCCAGGCTCACATA
SNAILAGGCCCTGGCTGCTACAAGTGCCCTCCCTCCACAGAAAT
ZEB-1TTGCCGCTGTTGCTGATGTGGTGCCCTGCCTCTGGTCCTC
E-CADHERINCTTCACCGACTTACCTACTGTGCCATACACTTAATTCTC
MMP1ATTCTACTGATATCGGGGCTTTGAATGTCCTTGGGGTATCCGTGTAG
MMP2TATTTGATGGCATCGCTCAGGCCTCGTATACCGCATCAAT
MMP3ATGCCCACTTTGATGATGATGAACCCACGCCTGAAGGAAGAGATG
MMP7TGCTCACTTCGATGAGGATGTGGGGATCTCCATTTCCATA
MMP9TTGACAGCGACAAGAAGTGGCCCTCAGTGAAGCGGTACAT
MMP10GAGTGGGGCAGCAAAAGAGGAGTGGGCCATCAAAAGAGTAAAAGTC
MMP13CTTCGGCTTAGAGGTGACTGGTTTTGCCGGTGTAGGTGTAGATAG
MMP14AGGCCGACATCATGATCTTCTTTGGGTGGGGTTTTTGGGTTTATCAGG
TIMP1ACCCCCGCCATGGAGAGTGTCCCGGAAGAAAGATGGGAGTG
TIMP2CGCAGCAAACACATCCGTAGAAGGCCGAGGAGGGGGCCGTGTAGATAA
TIMP3CCCTTCGGCACGCTGGTCTACATCAGCGGGAAGGGAGGGAAGTG
TIMP4CGTCGGGGCGGGATTGGACTGCTTCTGGCTGTTGGCTTCTA
Sequences were designed with Primer 3 software (http://primer3.sourceforge.net/) or with the DNASTAR Lasergene Suite (Madison, WI, United States). Sequences were checked against the human transcript database via PrimerBlast (http://www.ncbi.nlm.nih.gov/tools/primer-blast/) for cross-amplification of unintended transcripts. MMP: Matrix metalloproteinase; TIMP: Tissue inhibitor of metalloproteinase.
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