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©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Sep 7, 2014; 20(33): 11840-11849
Published online Sep 7, 2014. doi: 10.3748/wjg.v20.i33.11840
Published online Sep 7, 2014. doi: 10.3748/wjg.v20.i33.11840
Table 1 Identification of down-regulated proteins in SW480 cells treated with caffeic acid phenethyl ester
| No. | Protein name | Accession No. | Matched peptides | Protein sequence coverage | Mascot score | MW/pI |
| 1 | Triosephosphate Isomerase (Tim) | gi|15079533 | FFVGGNWK | 79% | 131 | 26.8/6.51 |
| KQSLGELIGTLNAAK | ||||||
| VPADTEVVCAPPTAYIDFAR | ||||||
| IAVAAQNCYK | ||||||
| VTNGAFTGEISPGMIK | ||||||
| DCGATWVVLGHSER | ||||||
| RHVFGESDELIGQK | ||||||
| HVFGESDELIGQK | ||||||
| VAHALAEGLGVIACIGEK | ||||||
| VVLAYEPVWAIGTGK | ||||||
| TATPQQAQEVHEK | ||||||
| SNVSDAVAQSTR | ||||||
| IIYGGSVTGATCK | ||||||
| ELASQPDVDGFLVGGASLKPEFVDIINAK | ||||||
| 2 | PSMA4 protein | gi|34783332 | TTIFSPEGR | 50% | 70 | 29.6/7.56 |
| LLDEVFFSEK | ||||||
| LNEDMACSVAGITSDANVLTNELR | ||||||
| YLLQYQEPIPCEQLVTALCDIK | ||||||
| RPFGVSLLYIGWDK | ||||||
| HYGFQLYQSDPSGNYGGWK | ||||||
| ATCIGNNSAAAVSMLK | ||||||
| QKEVEQLIK | ||||||
| KHEEEEAK | ||||||
| 3 | Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 | gi|5174447 | GHNGWVTQIATTPQFPDMILSASR | 80% | 154 | 35.5/7.6 |
| DETNYGIPQR | ||||||
| GHSHFVSDVVISSDGQFALSGSWDGTLR | ||||||
| LWDLTTGTTTR | ||||||
| DVLSVAFSSDNR | ||||||
| YTVQDESHSEWVSCVR | ||||||
| FSPNSSNPIIVSCGWDK | ||||||
| FSPNSSNPIIVSCGWDKLVK | ||||||
| VWNLANCK | ||||||
| TNHIGHTGYLNTVTVSPDGSLCASGGK | ||||||
| DGQAMLWDLNEGK | ||||||
| HLYTLDGGDIINALCFSPNR | ||||||
| YWLCAATGPSIK | ||||||
| IIVDELKQEVISTSSK | ||||||
| AEPPQCTSLAWSADGQTLFAGYTDNLVR | ||||||
| VWQVTIGTR | ||||||
| 4 | Phosphoserine aminotransferase 1 (PSAT1) | gi|17390289 | QVVNFGPGPAK | 38% | 79 | 40.9/7.56 |
| LPHSVLLEIQK | ||||||
| GVGISVLEMSHR | ||||||
| CADYVVTGAWSAK | ||||||
| FGTINIVHPK | ||||||
| FGTINIVHPKLGSYTK | ||||||
| GAVLVCDMSSNFLSKPVDVSK | ||||||
| NVGSAGVTVVIVR | ||||||
| DDLLGFALR | ||||||
| SQTIYEIIDNSQGFYVCPVEPQNR | ||||||
| ASLYNAVTIEDVQK | ||||||
| 5 | Proteasome (prosome, macropain) subunit, alpha type, 1 (PSMA1) | gi|18490859 | NQYDNDVTVWSPQGR | 50% | 81 | 29.8/6.15 |
| IHQIEYAMEAVK | ||||||
| IHQIEYAMEAVKQGSATVGLK | ||||||
| ILHVDNHIGISIAGLTADAR | ||||||
| LLCNFMR | ||||||
| FVFDRPLPVSR | ||||||
| HMSEFMECNLNELVK | ||||||
| DLEFTIYDDDDVSPFLEGLEERPQR | ||||||
| AQPAQPADEPAEKADEPMEH | ||||||
| 6 | Myosin XVIIIB | gi|51317366 | DRQGTRPQAQGPGEGVRPGK | 14% | 75 | 28.7/6.45 |
| EGAEPTNTVEKGNVSK | ||||||
| STTGKAGESWDK | ||||||
| MGQPQGKSGNAGEAR | ||||||
| AGDGAGALETELEGPSQPALEK | ||||||
| AGDGAGALETELEGPSQPALEKDAERPR | ||||||
| RDQSIVALGWSGAGK | ||||||
| QKAAAAFAQLQGAMEMLGISESEQR | ||||||
| AAAAFAQLQGAMEMLGISESEQRAVWR | ||||||
| QIIQQMTFGPSR+ Oxidation (M) | ||||||
| SFSSHHLSMASIMVVDSPGFQNPR + 2 Oxidation (M);Pyro-glu (N-term Q) | ||||||
| LQLLFYQRTFVSTLQR | ||||||
| AVAGLEGTSQQALQR | ||||||
| LQMDALTSMIK+ 2 Oxidation (M) | ||||||
| NPTGGADEWQMR + 2 Oxidation (M) | ||||||
| FDLQLAQALGESVFEK | ||||||
| WELGQLQQQLKQK | ||||||
| FELEIERMK+ Oxidation (M) | ||||||
| RTHALLSDVQLLLGTMEDGK | ||||||
| HKLQEQLQVAQMR+ Oxidation (M) | ||||||
| DSLIKMGEELSQAATSESQQR+ Oxidation (M) | ||||||
| CMELEKYVEELAAVR + Oxidation (M) | ||||||
| INEEAGDTERTQSALALSR + Oxidation (M) | ||||||
| DMLLSPTLRPR+ Oxidation (M) | ||||||
| DMLLSPTLRPRR | ||||||
| 7 | Chain B, A Short Peptide Insertion Crucial For Angiostatic Activity Of Human Tryptophanyl-tRNA Synthetase | gi|42543731 | EDFVDPWTVQTSSAKGIDYDK | 56% | 79 | 44.9/6.41 |
| ATGQRPHHFLR | ||||||
| GIFFSHR | ||||||
| GIFFSHRDMNQVLDAYENK | ||||||
| KPFYLYTGR | ||||||
| GPSSEAMHVGHLIPFIFTK | ||||||
| WLQDVFNVPLVIQMTDDEK+ Oxidation (M) | ||||||
| TFIFSDLDYMGMSSGFYK + Oxidation (M) | ||||||
| HVTFNQVK | ||||||
| GIFGFTDSDCIGK | ||||||
| ISFPAIQAAPSFSNSFPQIFR | ||||||
| IGYPKPALLHSTFFPALQGAQTK | ||||||
| MSASDPNSSIFLTDTAK | ||||||
| ALIEVLQPLIAEHQAR | ||||||
| KLSFDFQ |
Table 2 Identification of up-regulated proteins in SW480 cells treated with caffeic acid phenethyl ester
| No. | Protein name | Accession No. | Matched peptides | Protein sequence coverage | Mascot score | MW/pI |
| 8 | Annexin A4 | gi|12652859 | AASGFNAMEDAQTLR | 56% | 105 | 36.2/5.65 |
| GLGTDEDAIISVLAYR | ||||||
| GAGTDEGCLIEILASR | ||||||
| ISQTYQQQYGR | ||||||
| SLEDDIRSDTSFMFQR + Oxidation (M) | ||||||
| SDTSFMFQR+ Oxidation (M) | ||||||
| VLVSLSAGGR | ||||||
| DEGNYLDDALVR | ||||||
| QDAQDLYEAGEK | ||||||
| FLTVLCSR | ||||||
| NRNHLLHVFDEYK | ||||||
| NHLLHVFDEYK | ||||||
| NHLLHVFDEYKR | ||||||
| SETSGSFEDALLAIVK | ||||||
| NKSAYFAEK | ||||||
| GLGTDDNTLIR | ||||||
| VMVSRAEIDMLDIR+2 Oxidation (M) | ||||||
| AEIDMLDIR | ||||||
| 9 | Glyceraldehyde-3-phosphate dehydrogenase | gi|31645 | LVINGNPITIFQERDPSK | 46% | 76 | 36.2/8.28 |
| WGDAGAEYVVESTGVFTTMEK | ||||||
| RVIISAPSADAPMFVMGVNHEK | ||||||
| IISNASCTTNCLAPLAK | ||||||
| VIHDNFGIVEGLMTTVHAITATQK | ||||||
| GALQNIIPASTGAAK | ||||||
| VPTANVSVVDLTCR | ||||||
| LISWYDNEFGYSNR | ||||||
| VVDLMAHMASK+2 Oxidation (M) | ||||||
| 10 | Glucosamine-6-phosphate deaminase 1 (GNPDA1) | gi|18490843 | IIQFNPGPEK | 54% | 90 | 32.8/6.42 |
| YFTLGLPTGSTPLGCYK | ||||||
| TFNMDEYVGLPR | ||||||
| AAGGIELFVGGIGPDGHIAFNEPGSSLVSR | ||||||
| TLAMDTILANAR | ||||||
| VPTMALTVGVGTVMDAR | ||||||
| EVMILITGAHKAFALYK | ||||||
| AIEEGVNHMWTVSAFQQHPR | ||||||
| TVFVCDEDATLELK | ||||||
| ETEKSQSSK | ||||||
| 11 | Glutathione peroxidase 1 (GPX1) | gi|14717805 | GLVVLGFPCNQFGHQENAK | 56% | 70 | 22.2/6.15 |
| YVRPGGGFEPNFMLFEK | ||||||
| CEVNGAGAHPLFAFLR | ||||||
| EALPAPSDDATALMTDPKLITWSPVCR | ||||||
| LITWSPVCR | ||||||
| FLVGPDGVPLR | ||||||
| FLVGPDGVPLRR | ||||||
| RFQTIDIEPDIEALLSQGPSCA |
- Citation: He YJ, Li WL, Liu BH, Dong H, Mou ZR, Wu YZ. Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester. World J Gastroenterol 2014; 20(33): 11840-11849
- URL: https://www.wjgnet.com/1007-9327/full/v20/i33/11840.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i33.11840