Relationship between pouch microbiota and pouchitis following restorative proctocolectomy for ulcerative colitis

Table 1 Studies on the pouch microbiota in which molecular methods were used

Ref.	Year	Microbiota identification	Sampling andanalysis	Normal pouch (n)	Pouchitis(n)	Bacteria normalpouch	Bacteria pouchitis	Taxonomy level	Methods	Database
Komanduri et al[89]	2007	16S rRNA	Mucosa adherent microbiota	15	5	Clostridium paraputrificum	Fusobacterium varium	Genus and species	LH-PCR	PCO plot
		Technique (LH–PCR)				E. coli/Shigella ssp.	Ruminococcus obeum
Lim et al[21]	2009	16S rDNA PCR products	Stool microbiota	15	5	No differences		Genus and species	T-RFLP	BLAST
McLaughlin et al[49]	2010	16S rDNA PCR products	Mucosal adherent microbiota	8	8	No differences		Phylum , family and species	PCR	Mega BLAST
Zella et al[45]	2011	16S rRNA PCR products	Stool microbiota	3	9	Bacteroidetes (vs FAP)	Clostridium	Phylum, Family Genus, species	RFLP	BLAST
							Eubacterium
							Firmicutes (vs FAP)
Scarpa et al[58]	2011	16S rDNA PCR products	Mucosa adherent microbiota culture	22	10	Enterobacteriaceae	Clostridiaceae	Family	GAPDH, qRT-PCR	BLAST
						Enterococcaceae	Bacteroidaceae
Tyler et al[90]	2013	16S rDNA PCR products	Mucosa adherent microbiota	19	15	Bacteroides	Proteobacteria	Phylum, genus	qPCR	Mothur
						Sutterella	Firmicutes
						Blautia	Roseburia spp.
						Moryella	Eubacterium rectale
						Dorea
						Parabacteroides

FAP: Familial adenomatous polyposis. E. coli: Escherichia coli; LH-PCR: Length Heterogeneity Polymerase Chain Reaction.