Mast cell deficiency exacerbates inflammatory bowel symptoms in interleukin-10-deficient mice

Table 1 Primer sets used for quantitative reverse transcriptase-polymerase chain reaction analysis of mouse colonic tissue

Gene name	Accession No.	Product size	Direction	Sequence (5’ to 3’)	Ref.
Claudin2	NM_016675.4	120 bp	Forward	GGCGTCCAACTGGTGGGCTAC	[42]
			Reverse	AACCGCCGTCACAATGCTGGC
Claudin3	NM_009902.4	132 bp	Forward	CAGGGGCAGTCTCTGTGCGAG	[42]
			Reverse	GCCGCTGGACCTGGGAATCAAC
GAPDH	NM_008084.2	132 bp	Forward	AACTTTGGCATTGTGGAAGG	[42]
			Reverse	GGATGCAGGGATGATGTTCT
IL-1β	NM_008361	73 bp	Forward	TCGCTCAGGGTCACAAGAAA	[43]
			Reverse	CATCAGAGGCAAGGAGGAAAAC
INFγ	NM_008337.3	93 bp	Forward	AGGTCCAGCGCCAAGCATTCAA	[42]
			Reverse	AGCAGCGACTCCTTTTCCGCTT
iNOS	U43428.1	76 bp	Forward	CAAAGTCTCAGACATGGCTTGC	This study
			Reverse	TTCCTCTGTCAGGTCACTTTGG
NOX1	NM_172203.1	113 bp	Forward	CAGGCATCCTCATTTTGCGG	This study
			Reverse	CCTTCTGCTGGGAGCGATAA
T-bet	NM_019507.2	138 bp	Forward	CCACTGGATGCGCCAGGAAGTT	[42]
			Reverse	TTCACCTCCACGATGTGCAGCC
TNF-α	NM_013693.2	67 bp	Forward	TGGGACAGTGACCTGGACTGT	[43]
			Reverse	TTCGGAAAGCCCATTTGAGT

Table 2 Primers for quantitative polymerase chain reaction analysis of selected fecal microbiota

Target organism	Primer set	Sequence (5’ to 3’)	Product size	Ref.
Bacteroides	BactF285	GGTTCTGAGAGGAGGTCCC	53	[44]
	UniR338	GCTGCCTCCCGTAGGAGT
Ec-ssul	Ec-ssu1F	GGATAACACTTGGAAACAGG	115	[45]
	Ec-ssu1R	TCCTTGTTCTTCTCTAACAA
Eubacteria	UniF340	ACTCCTACGGGAGGCAGCAGT	210	[46]
	UniR514	ATTACCGCGGCTGCTGGC
Lactobacillus	LabF362	AGCAGTAGGGAATCTTCCA	315	[44]
	LabR677	CACCGCTACACATGGAG
Ruminococcus albus (Ralb)	Ralb561F	CAGGTGTGAAATTTAGGGGC	246	[47]
	Ralb807R	GTCAGTCCCCCCACACCTAG

Table 3 Organ weights of 10-week-old interleukin-10-deficient and double knockout mice at necropsy

Organ weight (g)	IL-10-deficient	DKO	P value
Liver	1.12 ± 0.041	1.26 ± 0.03	0.0098
Heart	0.13 ± 0.01	0.13 ± 0.01	NS
Spleen	0.09 ± 0.01	0.16 ± 0.02	0.0006
Vastus muscle	0.24 ± 0.01	0.23 ± 0.02	NS
Gastrocnemius muscle	0.27 ± 0.01	0.25 ± 0.01	NS
Tibials muscle	0.081 ± 0.003	0.076 ± 0.003	NS
Subcutaneous fat	0.31 ± 0.03	0.21 ± 0.02	0.0052
Gonadal fat	0.33 ± 0.04	0.27 ± 0.03	NS

¹Mean ± SE, n = 10. IL: Interleukin; DKO: Double knockout.