Expression of P450 and nuclear receptors in normal and end-stage Chinese livers

Table 1 Sample demographics (n = 128)

	Liver disease	Gender		Age (yr)
		Men	Women	21-40	40-60	61-80
Normal donors	Trimmed normal livers	32	3	34	1	0
End-stage livers	HCC	22	2	4	16	4
	Peri-HCC	22	2	4	16	4
	HBV cirrhosis	21	6	2	18	7
	Severe cirrhosis	11	2	4	7	2
	Alcoholic cirrhosis	5	0	0	5	0

HCC: Hepatocellular carcinoma; HBV: Hepatitis B virus.

Table 2 Primer sequence for real-time RT-PCR analysis

Gene	GenBank	Forward	Reverse
	number
AhR	NM_001621	CTGCCTTTCCCACAAGATGT	AGTTATCCTGGCCTCCGTTT
CAR	NM_005122	CTTCTCTCCTGACCGACCTG	AGGCCTAGCAACTTCGCATA
CYP1A2	NM_000761	GGACAGCACTTCCCTGAGAG	GAGGCAGTCTCCACGAACTC
CYP2B6	NM_000767	TCCAGTCTCAGCTCCCAAGT	CTGGCCAACATGTCCCTACT
CYP2C9	NM_000771	CCACATGCCCTACACAGATG	TGCCCTTGGGAATGAGATAG
CYP2C19	NM_000769	CAACAACCCTCGGGACTTTA	GTCTCTGTCCCAGCTCCAAG
CYP2D6	NM_000106	CAGAGATGGAGAAGGCCAAG	AGAACAGGTCAGCCACCACT
CYP2E1	NM_000773	CCCAAAGGATATCGACCTCA	AGGGTGTCCTCCACACACTC
CYP3A4	NM_017460	ACCGTGACCCAAAGTACTGG	GTTTCTGGGTCCACTTCCAA
CYP3A5	NM_000777	GGAGATGTTCCCCATCATTG	GCCCCAAAGATGTCTTTCAA
CYP3A7	NM_000765	GAAACACAGATCCCCCTGAA	AGGGAAATCAGGCTCCACTT
CYP4A11	NM_000778	CACCACAACCCAAAAGTGTG	ATTGTTTCCCGATGCAGTTC
FXR	NM_005123	ATTTTGACGGAAATGGCAAC	AGACCCTTTCAGCAAAGCAA
GADPH	NM_002046	ACAGTCAGCCGCATCTTCTT	ACGACCAAATCCGTTGACTC
PPARα	Y07619	ACGATTCGACTCAAGCTGGT	GTTGTGTGACATCCCGACAG
PXR	NM_005123	GGAATGTTGGCTGAATGCTT	CTGCATGCTGCTTCACATTT

Table 3 mRNA expression of Cytochrome P450 enzymes and corresponding nuclear receptors

	Normal liver (n = 35)			End-stage livers (n = 93)
Gene	Expression%	-fold	mean ± SE	Expression%	-fold	mean ± SE
AhR	5.52-27.1	4.91	14.7 ± 0.74	2.71-128	47.2	17.4 ± 1.52
CYP1A2	20.5-537	26.2	234 ± 94.6	0.78-175	224	136 ± 23.9a
CAR	0.55-14.1	25.6	7.87 ± 0.50	0.21-293	1395	7.73 ± 0.86
CYP2B6	1.31-102	77.9	17.1 ± 2.74	0.81-128	158	27.2 ± 2.77a
CYP2C9	14.4-1544	107	786 ± 57.9	3.74-1486	387	418 ± 34.8a
CYP2C19	49.6-1254	25.2	594 ± 40.2	1.75-1379	788	256 ± 23.9a
CYP2D6	10.7-316	29.5	149 ± 12.2	4.31-930	216	156 ± 15.6
CYP2E1	210-3577	17.2	1708 ± 115	17.7-4373	247	990 ± 75.4a
PXR	14.2-189	13.3	41.4 ± 4.95	3.01-87.1	29.0	33.0 ± 1.81
CYP3A4	171-1357	7.94	798 ± 49.2	8.21-1966	240	498 ± 48.6a
CYP3A5	22.2-659	30.0	278 ± 26.9	9.92-979	98.7	203 ± 19.1a
CYP3A7	86.3-1054	12.2	450 ± 36.7	1.51-2137	1415	479 ± 47.7
PPARα	13.7-57.7	4.21	29.4 ± 1.89	2.61-169	64.8	27.6 ± 2.43
CYP4A11	15.1-478	31.6	198 ± 17.2	1.41-740	524	89.2 ± 11.1a

Relative transcript levels (% of GAPDH), “-fold” indicates the inter-individual variations within normal or end-stage livers. Significantly different from normal livers,

^aP < 0.05.