Protective effect of bone marrow mesenchymal stem cells in intestinal barrier permeability after heterotopic intestinal transplantation

doi:10.3748/wjg.v20.i23.7442

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 21, 2014; 20(23): 7442-7451
Published online Jun 21, 2014. doi: 10.3748/wjg.v20.i23.7442

Table 1 Sequences of primers for occludin, zona occludens-1, and β-actin

Tested gene product		Primer sequences	Fragment
Occludin	Upstream	5’-CCTGTTTAGTTAGGTGAAG-3’	156 bp
Occludin	Downstream	5’-TTCCTGAGAAGGGTTATG-3’	156 bp
ZO-1	Upstream	5’-GGGGGATTTATAACTTGGG-3’	321 bp
ZO-1	Downstream	5’-CTGGTTGGATGTCTGTGG-3’	321 bp
β-Actin	Upstream	5’-GCGTGACATTAAAGAGAAGCTG-3’	500 bp
β-Actin	Downstream	5’-AGAAGCATTTGCGGTGCAC-3’	500 bp

ZO-1: Zona occludens-1.

Table 2 Grade of intestinal mucosal injury following heterotopic intestinal transplantation

Group	D 1	D 3	D 5	D 7	D 10
A	8.26 ± 0.65	9.37 ± 0.92	10.36 ± 0.78	12.65 ± 0.65	13.14 ± 0.86
B	17.4 ± 1.76^a	30.64 ± 3.62^a	41.6 ± 2.27^a	67.2 ± 2.57^a	81.74 ± 3.52^a
C	12.17 ± 1.17^c	24.00 ± 1.54^c	30.17 ± 0.41^c	41.83 ± 2.93^c	52.33 ± 1.03^c

All values are mean ± SD (n = 6; the summation of six randomly chosen fields from each rat were evaluated and averaged to determine the degree of mucosal injury). A: Non-rejection group; B: Rejection group; C: Bone marrow mesenchymal stem cells therapy group.

^aP < 0.05 vs group A;

^cP < 0.05 vs group B.

Table 3 Levels of graft diamine oxidase and D-lactic acid following heterotopic intestinal transplantation

	Group	D 1	D 3	D 5	D 7	D 10
DAO (U/mL)	A	10.15 ± 1.10	12.86 ± 1.35	15.76 ± 1.33	18.55 ± 1.77	21.83 ± 1.21
	B	22.36 ± 2.82^a	34.74 ± 5.59^a	44.54 ± 2.77^a	51.61 ± 1.70^a	68.02 ± 2.46^a
	C	18.69 ± 2.17^c	29.79 ± 2.49^c	36.15 ± 3.98^c	40.13 ± 1.21^c	59.87 ± 4.34^c
D-LA (g/L)	A	4.12 ± 0.53	6.13 ± 0.57	8.62 ± 1.67	10.78 ± 0.72	11.67 ± 1.56
	B	6.32 ± 0.46^a	8.65 ± 0.62^a	10.46 ± 0.98^a	12.74 ± 0.68^a	17.74 ± 1.75^a
	C	3.08 ± 0.32^c	4.08 ± 0.30^c	5.031 ±0.18^c	7.25 ± 0.51^c	12.81 ± 0.47^c

All values are mean ± SD (n = 6). A: Non-rejection group; B: Rejection group; C: Bone marrow mesenchymal stem cells therapy group.

^aP < 0.05 vs group A;

^cP < 0.05 vs group B. DAO: Diamine oxidase; D-LA: D-lactic acid.

Citation: Zhang W, Shen ZY, Song HL, Yang Y, Wu BJ, Fu NN, Liu T. Protective effect of bone marrow mesenchymal stem cells in intestinal barrier permeability after heterotopic intestinal transplantation. World J Gastroenterol 2014; 20(23): 7442-7451
URL: https://www.wjgnet.com/1007-9327/full/v20/i23/7442.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i23.7442