Association of CD14/-260 polymorphism with gastric cancer risk in Highland Tibetans

Table 1 Primers and probes used for genotyping CD14-260C/T and -651C/T

Polymorphism	Primers and probes	Length, bp
-260 C/T	5'-CACCCACCAGAGAAGGCTTA-3'	212
	5'-ATCACCTCCCCACCTCTCTT-3'
Common probe	5'-CCCCCTCCCTGAAACATCCTTTTTTTTTTTTTTTTTTT-FAM-3'
Discriminating	260C/T-R_G	77
probes	5'-TTTTTTTTTTTTTTTTTGCAGAATCCTTCCTGTTACGGC
	260C/T-R_A-3'	79
	5'-TTTTTTTTTTTTTTTTTTTGCAGAATCCTTCCTGTTACGGT-3'
-651C/T	5'-GGGTAGAATTAGGTTCAAG-3'	103
	5'-CTTAATCAAAGGAGCAAGG-3'	1
Common probe	5'-GTCTAAAGAAAAATTCCCCCTTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT-FAM-3'
Discriminating probes	651C/T_C	150
	5'-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTTCAAGAAAAGGAAGTTG-3'
	651C/T_T	152
	5'-TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGTTCAAGAAAAGGAAGTTA-3'

Table 2 Characteristics of study subjects n (%)

Variable	Controls	GC patients	aP value
	n = 237	n = 225
Age, yr	54.8 ± 11.2	54.0 ± 12.3	NS
Male	175 (73.8)	172 (76.4)	NS
Clinical stage
I		7 (3.1)
II		21 (9.3)
III		126 (56.0)
IV		71 (31.6)

^aGC vs controls by Student’s t test or χ² test. GC: Gastric cancer; NS: Not significant.

Table 3 Genotype and allele frequencies ofCD14-260C/T and -651C/T in gastric cancer patients and healthy control subjects n (%)

Polymorphism	Case	Control	OR	95%CI	P value
	n = 225	n = 237
-260 C/T
Genotypes
CC	43 (19.1)	79 (33.3)	1.000	(reference)	-
CT	87 (38.7)	77 (32.5)	2.076	1.282-3.360	0.003
TT	95 (42.2)	81 (34.2)	2.155	1.340-3.466	0.001
Alleles
C	173 (38.4)	235 (49.6)	1.000	(reference)	-
T	277 (61.6)	239 (50.4)	1.574	1.121-2.045	0.001
-651 C/T
Genotypes
CC	145 (64.4)	134 (56.5)	1.000	(reference)	-
CT	66 (29.3)	83 (35.0)	0.735	0.493-1.096	0.130
TT	14 (6.2)	20 (8.4)	0.647	0.314-1.332	0.234
Alleles
C	356 (79.1)	351 (74.1)	1.000	(reference)	-
T	94 (20.9)	123 (25.9)	0.753	0.55-1.024	0.07

Table 4 Haplotype distribution of CD14-260C/T and -651 C/T in gastric cancer patients and healthy control subjects n (%)

Haplotypes -260C/T - -651 C/T	Case	Control	OR (95%CI)	P value
T-C	244 (57.9)	206 (46.5)	1.58 (1.21-2.07)	0.001
C-C	87 (20.8)	120 (27.2)	0.70 (0.51-0.96)	0.028
C-T	76 (18.1)	97 (21.9)	0.79 (0.56-1.10)	0.157
T-T	13 (3.3)	19 (4.4)	0.73 (0.36-1.47)	0.371

Global χ² is 11.44, degrees of freedom = 3, P = 0.009. Bolded values are statistically significant.