ATG16L1 and NOD2 polymorphisms enhance phagocytosis in monocytes of Crohn’s disease patients

Table 1 Clinical phenotypic characteristics of study population n (%)

	CD (n = 65)	UC (n = 34)
Gender: M/F	29/36
Montreal classification
Age at diagnosis (yr)	27 (9-68)
< 17	10 (15.4)
17-40	44 (67.7)
> 40	9 (13.8)
Disease localisation
Terminal ileum (L1)	17 (26.2)
Colonic (L2)	8 (12.3)
Ileocolonic (L3)	23 (35.4)
Upper gastrotractinal (L4)	0
Disease behavior
Non-stricturing /non-penetrating	20 (30.8)
Stricturing	15 (23.1)
Penetrating	11 (16.9)
Missing	19 (29.2)
Age at diagnosis (yr)	-	32 (19-74)
< 40	-	25 (73.5)
> 40	-	7 (20)
Disease localisation
Proctitis (L1)		3 (8.8)
Left sided (L2)		11 (32.4)
Pancolitis (L3)		10 (29.4)
Missing		10 (29.4)
Disease activity (Y/N)	17/47	14/10
Positive family history	12	8
Operated on	40	7

CD: Crohn’s disease; UC: Ulcerative colitis; M/F: Male/female; Y/N: Yes/no.

Table 2 Designed primers and restriction enzymes for genotyping by restriction fragment length polymorphisms

Gene	Forward	Reverse	Restriction enzyme
ATG16L1	GGTACCCTCACTTCTTTACCAGAA CCAGGAAGAG	TGGAGTCCACAGGTTAGTGTGCAGGAGAGTAAGG	Sap1
rs2241880
IRGM	CCCGTGTCGTACCCAAGCAGAGTGTGCTTGAAGA	CTTTACCATTGTACTCCTTGTGCCCAGCAGGTG	MboI
rs13361189
NOD2	ATGTGTCTAAGGGACAGGTG	AACTGAGGTTCGGAGAGCTA	NlaIV
rs2066847
rs5743293

rs2066847 and rs5743293 are the same single-nucleotide polymorphism (SNP) in nucleotide-binding ligomerization domain-containing protein 2 (NOD2). ATG16L1: Autophagy related like 1; IRGM: Immunity-related GTPase family M protein.