Detecting the localization of hepatitis B and C viruses in hepatocellular carcinoma by double in situ hybridization

Table 1 The background information of 10 cases of hepatitis B virus patients

Specimen number	Age	Sex	HBV serum marker	AFP (ρB/μg·L-1)	Pathological diagnosis
1	48	Male	HBsAg (+) HBeAb (+) HBcAb (+)	42	HCC, chronic hepatitis
2	27	Male	HBsAg (+)	900	HCC, hepatitis in porta tract
3	61	Male	HBsAg (+) HBcAb (+)	260	HCC, cirrhosis
4	38	Male	(-)	12000	HCC, cirrhosis
5	44	Male	HBeAb (+) HBcAb (+)	40	HCC, cirrhosis
6	46	Male	(-)	150	HCC, non-cirrhosis
7	45	Male	(-)	< 25	HCC, cirrhosis
8	62	Male	(-)	52	HCC, cirrhosis
9	40	Male	HBsAg (+)	-	HCC, cirrhosis
10	58	Male	-	< 25	HCC, cirrhosis

HBV: Hepatitis B virus; AFP: Alpha fetal protein; HCC: Hepatocellular carcinoma.

Table 2 Results of single in situ hybridization

Specimen number	Dig-HBV		Dig-HCV
	C	S	C	S
1	+	+	-	-
2	+	+	+	+
3	+	+	-	-
4	+	+	+	+
5	+	+	-	-
6	-	-	-	-
7	+	+	-	-
8	+	+	-	-
9	+	+	-	-
10	+	+	-	-

C: Cancer; S: Tumor surrounding tissues; HBV: Hepatitis B virus; HCV: Hepatitis C virus.

Table 3 Results of double in situ hybridization of Dig-HBV and Bio-HCV

Specimen number	Dig-HBV		Bio-HCV
	C	S	C	S
1	+	+	-	-
2	+	+	+	+
3	+	+	-	-
4	+	+	+	+
5	+	+	-	-
6	-	-	-	-
7	+	+	-	-
8	+	+	-	-
9	+	+	-	-
10	+	+	-	-

C: Cancer; S: Tumor surrounding tissues; HBV: Hepatitis B virus; HCV: Hepatitis C virus.