Anti-rejection therapy with tripterygium woifordii and low-dose cyclosporine for small bowel transplantation in pigs

Table 1 Technical failures

Group	Animal No.	Survival days	Cause of death
I	1	5	Venous thrombosis
	3	1	Venous thrombosis
	5	0	Anesthetic
	9	1	Transplant intussusception
	10	32	Anesthetic, Rejection?
II	11	1	Venous thrombosis
	13	4	Transplant intussusception
	16	4	Arterial thrombosis
	17	7	Arterial thrombosis
III	19	1	Venous thrombosis
	22	0	Anesthetic
	26	0	Anesthetic
IV	29	3	Venous thrombosis
	31	4	Venous thrombosis
	33	6	Arterial thrombosis

Table 2 Immunosuppression and survival

Group	Immunosuppressant	Animal No.	Survival days	Cause of death
I	None	2	14	Rejection
		4	10	Rejection
		6	10	Rejection
		7	16	Rejection
		8	14	Rejection
II	High-dose CsA	12	92	Infection
		14	398	Survival
		15	97	Infection
		18	> 419	Survival
III	Tw + low-dose CsA	20	> 274	Survival
		21	> 356	Survival
		23	166	Ileus
		24	135	Unknown
		25	> 285	Survival
IV	Low-dose CsA	27	16	Rejection
		28	14	Rejection
		30	10	Rejection
		32	10	Rejection
		34	12	Rejection

TW: Tripterygium woilfordii.

Table 3 Mean survival period with or without percutaneous transhepatic cholangial drainage prior to operation

Group, n	5	10	20	40	50	80	60	100
Group II, 4	1476 ± 11.6b	902 ± 79b	894 ± 89b	896 ± 86b	878 ± 87b	495 ± 109b		-425, 388
Group III, 5	429 ± 79	304 ± 33	298 ± 34	301 ± 39	289 ± 36	157 ± 30	155 ± 35
Group IV, 5	446 ± 63	304 ± 48

^bP < 0.01 vs group III and IV.

Table 4 Results of liver kidney functions in Groups II and III

Biochemistry	Preoperative	POD 40		POD 80
	Control	Group II	Group III	Group II	Group III
ALT (nkat)	280 ± 40	429 ± 89a	350 ± 53	629 ± 257a	327 ± 87
AST (nkat)	410 ± 90	771 ± 119a	690 ± 132	1267 ± 444a	410 ± 114
LDH (U/L)	301 ± 72	445 ± 69	370 ± 79	567 ± 147a	333 ± 140
AKP (nkat)	840 ± 184	942 ± 319a	837 ± 177	1304 ± 593	750 ± 196
Bil (μmol/L)	11.3 ± 5.5	17.1 ± 7.4	16.1 ± 5.7	31.2 ± 23.7	14.7 ± 7.4
BUN (mmol/L)	3.2 ± 0.8	3.2 ± 0.4	3.7 ± 1.1	4.3 ± 0.7	3.4 ± 0.8
Cr (μmol/L)	89 ± 26	28 ± 34	95 ± 27	93 ± 27	83 ± 20

^aP < 0.05 vs preoperative and group III. POD: Postoperative days.

Table 5 Results of serum biochemistry in group IV

Biochemistry	Preoperative	Postoperative days
	(Control)	130	160	190
Sodium (mmol/L)	153 ± 8	150 ± 8	153 ± 10	153 ± 9
Potassium (mmol/L)	5.7 ± 1.0	5.9 ± 0.9	5.9 ± 1.0	5.8 ± 1.1
Chloride (mmol/L)	101 ± 5	100 ± 4	101 ± 5	102 ± 3
Total protein (g/L)	87.8 ± 6.8	85.3 ± 8.1	84.3 ± 4.1	85.0 ± 5.1
Albumin (g/L)	37.2 ± 5.9	36.0 ± 3.3	37.0 ± 2.8	37.0 ± 2.2
Globulin (g/L)	50.6 ± 4.4	49.3 ± 5.5	47.3 ± 2.2	48.0 ± 5.4
A/G	0.74 ± 0.14	0.74 ± 0.06	0.78 ± 0.06	0.78 ± 1.1
ALT (nkat)	280 ± 40	304 ± 37	283 ± 101	300 ± 53
AST (nkat)	410 ± 90	496 ± 184	450 ± 175	484 ± 168
LDH (u/L)	301 ± 72	289 ± 65	273 ± 66	278 ± 74
AKP (nkat)	840 ± 184	859 ± 97	805 ± 104	824 ± 127
Bilirubin (μmol/L)	11.3 ± 5.5	13.5 ± 5.3	12.4 ± 5.1	12.6 ± 4.3
BUN (mmol/L)	3.2 ± 0.8	3.5 ± 0.8	3.4 ± 0.9	3.5 ± 0.9
Cr (μmol/L)	89 ± 26	88 ± 17	86 ± 22	92 ± 28

Survival > 200 d (n = 5).