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©2013 Baishideng Publishing Group Co.
World J Gastroenterol. Feb 14, 2013; 19(6): 946-950
Published online Feb 14, 2013. doi: 10.3748/wjg.v19.i6.946
Published online Feb 14, 2013. doi: 10.3748/wjg.v19.i6.946
Table 1 Primer pairs used for amplification of ABCC2/MRP2 gene exons and promoters
| Pair No. | Sequences of polymerase chain reaction primer pairs used to amplify the ABCC2/MRP2 gene from genomic DNA | Exon No. | |
| Forward primer | Reverse primer | ||
| 1 | 5=-TTTACAATGCCTGGCAAAGG-3= | 5=-CAGCATGATTCCTGGACTGC-3= | Promoter |
| 2 | 5=-TCCCACATTCTGGATTTTGAC-3= | 5=-ATAAAATAGCTTATCCAGTGTGAGC-3= | Promoter |
| 3 | 5=-GGTCAGACCAATTTACATTTCCATC-3= | 5=-CATAACCACCCATGCAGTATCC-3= | Promoter |
| 4 | 5=-TACTTTGGGAACTGGTGAGTCT-3= | 5=-AGAAGGCAATTTTGCGACTA-3= | 1 |
| 5 | 5=-CACAAATAGGAAAATACGGATA-3= | 5=-CCTGGGACAGCTGCTTA-3= | 2 |
| 6 | 5=-CTGAATCACTGCATACCGCTTTT-3= | 5=-CCAACCAAGCTTTGCCTCAC-3= | 3 |
| 7 | 5=-CTGAATCACTGCATACCGCTTTT-3= | 5=-AATTCGATCCTGGAGCTCAAC-3= | 3_4 |
| 8 | 5=-TCATAGTAAATGGCATCAAGT-3= | 5=-GGTGGAACATGAGCTTGAGT-3= | 5_6 |
| 9 | 5=-AGTGGTGGAGATAGCCTC-3= | 5=-GCTATAAAAATGTAAGGACA-3= | 7 |
| 10 | 5=-GCCAGGGAGAGATGATCAAA-3= | 5=-GGCCAGTCAACATTAAGTG-3= | 8_9 |
| 11 | 5=-TGGAGCACATCCTTCCATTG-3= | 5=-TTGCCCAAACTCCCATTAAG-3= | 10 |
| 12 | 5=-TCACTGGGCACCTCAAGTTC-3= | 5=-AGCAGGAATCCATCACCTCT-3= | 11 |
| 13 | 5=-ATTTTGGGGACTATATCT-3= | 5=-GATGTGATAGCCAGTCATTC-3= | 12_13 |
| 14 | 5=-GTTCGGTGGAGATTAGGAG-3= | 5=-TCTTATGCAAGCATAGGCTC-3= | 14 |
| 15 | 5=-TTCACCTCCTGTTAGCGTA-3= | 5=-ACCGAAGACATGCACATAGC-3= | 15 |
| 16 | 5=-TTCACCTCCTGTTAGCGTA-3= | 5=-CAAGACCTCACCTACTAGCC-3= | 15_16 |
| 17 | 5=-ACAAGCACGTGAATACATATCAG-3= | 5=-ACCCCTGTGTAGTTCTT-3= | 17 |
| 18 | 5=-GTAAGATTTTTAACCCCTTG-3= | 5=-GCCCAGGCATAGAGTTTC-3= | 18_19 |
| 19 | 5=-GTATGGAGTATTTATGGAGT-3= | 5=-TGTAAGTATGCGTTCAAT-3= | 20_21 |
| 20 | 5=-GTGGTTGGCATTCTAGGT-3= | 5=-CATAATAATTCCTCCCTATCA-3= | 22_23 |
| 21 | 5=-CTGGGAACACACAGAATCCAAC-3= | 5=-GGCTCCTGGGTATGTCAACA-3= | 24 |
| 22 | 5=-GGCTTTTGTCTTGTTCAGACG-3= | 5=-CTTGGTAAACGGCAGA-3= | 25 |
| 23 | 5=-CCCGATCAAGTCAAAC-3= | 5=-GGCATTCATGTCTACTTAGGA-3= | 26 |
| 24 | 5=-GGAGGCAAGGATTGTC-3= | 5=-TCTGCATACTGTGGACCTTAT-3= | 27_28 |
| 25 | 5=-ACAGCTGCCAAGAGAGTCCAT-3= | 5=-GCTCAAGTATCCCGAGTAGA-3= | 29 |
| 26 | 5=-CCTTGCGGAAGCTCAACC-3= | 5=-TGCCAGGCATCACCTAACACG-3= | 30 |
| 27 | 5=-GTTTTGAAAGTCTGATCTG-3= | 5=-AGGAAGTACGATCGAGGTA-3= | 31_32 |
- Citation: Sticova E, Elleder M, Hulkova H, Luksan O, Sauer M, Wunschova-Moudra I, Novotny J, Jirsa M. Dubin-Johnson syndrome coinciding with colon cancer and atherosclerosis. World J Gastroenterol 2013; 19(6): 946-950
- URL: https://www.wjgnet.com/1007-9327/full/v19/i6/946.htm
- DOI: https://dx.doi.org/10.3748/wjg.v19.i6.946