Clinicopathological and biological significance of cripto overexpression in human colon cancer

Table 1 Clinicopathologic characteristics of colon cancer patients n (%)

Characteristic	No. of patients
Sex
Male	21 (53.8)
Female	18 (46.2)
Age (yr), mean (range)	62 (35–81)
≤ 65	20 (51.3)
> 65	19 (48.7)
Dukes staging
A + B	12 (30.8)
C + D	27 (69.2)
Tumor differentiation
Well	0
Moderate	29 (74.4)
Poor	10 (25.6)

Table 2 Primers of cripto, matrix metalloproteinase-7 and matrix metalloproteinase-9

Genes	Forward(5'-3')	Reverse(5'-3')
Cripto	CAATTCGGCCTCGGTCTTC	TTCAGGCAGCAGGTTCTGTTT
MMP-7	AACTCCCGCGTCATAGAAAT	GATACGATCCTGTAGGTGAC
MMP-9	CGGAGTGAGTTGAACCAG	GTCCCAGTGGGGATTTAC

MMP: Matrix metalloproteinase.

Table 3 Cripto siRNA sequence

siRNA	Sense (5'→3')	Antisense (5'→3')	MW	Position
S1	UUCGGCCUCGGUCUUCCCATT	UGGGAAGACCGAGGCCGAATT	13347.2	175-195
S2	CAGAACCUGCUGCCUGAAUTT	AUUCAGGCAGCAGGUUCUGTT	13317.2	236-256
S3	CUGUGAGCACGAUGUGCGCTT	GCGCACAUCGUGCUCACAGTT	13347.2	314-334
S4	GAGAACUGUGGGUCUGUGCTT	GCACAGACCCACAGUUCUCTT	13332.2	336-356
S5	UGCUGGCACGGUCAGCUCCTT	GGAGCUGACCGUGCCAGCATT	13362.2	396-416
S6	CUACCACCGUCUGCACGUATT	UACGUGCAGACGGUGGUAGTT	13332.2	495-515