Cdx2 expression and its promoter methylation during metaplasia-dysplasia-carcinoma sequence in Barrett's esophagus

Table 1 Primer sequences used in polymerase chain-based assays, product size and annealing temperature

	Primer sequence	Size (bp)	Temp (°C)
First PCR	(F)5’-GTTAAGGGGTTTAGGGTTGGA	183	60
Nested PCR	(R)5’-CAAAAACTTATATTAAAAAATAAAC
Methylated	(F) 5’-GGAGTTGTTTCGATAGGAGCGC	71	60
	(R) 5’-TTACTAAAACCGAACTAAACGCG
Unmethylated	(F) 5’-GGAGTTGTTTTGATAGGAGTGT	71	60
	(R) 5’-TTACTAAAACCAAACTAAACACA

Temp: Temperature; PCR: Polymerase chain reaction; R: Reverse; F: Forward.

Table 2 Summary of immunohistochemical findings

	G-type	I-type (IM)	HD or IMC	PDA
MUC2	-	+	-	-
MUC5AC	+	-	++	+
MUC6	+	-	++	+
p53	-	-	++	++
E-cadherin	++	++	++	+
Cdx2	-	+	-	+

G-type: Gastric metaplasia; IM: Intestinal metaplasia; HD or IMC: High-grade dysplasia or intramucosal adenocarcinoma; PDA: Poorly-differentiated invasive adenocarcinoma; MUC: Mucin.