Alteration in gene expression profile and oncogenicity of esophageal squamous cell carcinoma by RIZ1 upregulation

Table 1 Sample qualification

Sample number	Concentration	A260/A280	2100 result		Results
	(μg/μL)		RIN	28S/18S
1	0.816	1.94	8.9	1.5	Qualified
2	0.865	1.93	8.9	1.5	Qualified

Table 2 Gene ontology analysis of differentially expressed genes

GO ID	Name	Hits	Total	Percentage	P value
GO: 0048468	Cell development	81	832	9.74%	0.0288
GO: 0050792	Regulation of viral reproduction	5	20	25.00%	0.0304
GO: 0031294	Lymphocyte costimulation	3	8	37.50%	0.0397
GO: 0002520	Immune system development	38	358	10.61%	0.0428

Hits: Number of differentially expressed genes in the pathway; Total: The total number of genes in the pathway; Percentage: Percentage hits in the pathway (Hits/Total); P: Enrichment P value; GO: Gene ontology.

Table 3 RIZ1 pathway analysis (selected pathways)

Name	Hits	Total	Percentage	P value	q
Cytokine-cytokine receptor interaction	25	276	9.06%	0	0
TGF-beta signaling pathway	10	85	11.76%	3.00E-04	5.00E-04
MAPK signaling pathway	20	271	7.38%	2.00E-04	5.00E-04
Pathways in cancer	20	328	6.10%	0.0018	0.0015

Hits: Number differentially expressed genes in the pathway; Total: Total number of genes in the pathway; Percentage: Percentage of hits in the pathway (Hits/Total); P: Enrichment P value; q: False discovery rate; TGF: Transforming growth factor; MAPK: Mitogen-activated protein kinase.