Original Article
Copyright ©2013 Baishideng Publishing Group Co.
World J Gastroenterol. Sep 21, 2013; 19(35): 5813-5827
Published online Sep 21, 2013. doi: 10.3748/wjg.v19.i35.5813
Table 1 Epidemiological profile of the population studied
Province/city of residenceTotal number of individualsMale/female numberAge1 (yr), mean ± SE
Buenos Aires/C.A.B.A.1461685/776a 33.4 ± 0.3 (11.2, 30)d
Catamarca648267/381 39.3 ± 0.5 (13.0, 38)d
Córdoba1061393/668b37.1 ± 0.4 (12.5, 35)
Chaco353175/178b 40.2 ± 0.8 (14.1, 40)d
Chubut17283/8937.4 ± 0.9 (12.1, 37)
Entre Ríos474225/24938.5 ± 0.5 (11.6, 38)
Jujuy176105/71b 35.2 ± 0.8 (10.3, 34)d
Río Negro329149/180 40.1 ± 0.7 (12.8, 39)d
Salta561230/331 41.3 ± 0.5 (12.8, 41)d
San Luis19552/143b 41.6 ± 1.2 (16.3, 40)d
Santiago del Estero375164/211b38.0 ± 0.7 (13.2, 35)
Tucumán446209/23737.7 ± 0.6 (12.0, 35)
Total62512738/351337.5 ± 0.2 (12.7, 35)
1Data are expressed as mean ± SE (SD, median).
aP < 0.05,
bP < 0.01, regarding the gender distribution (male/female ratio) within the whole population studied;
dP < 0.01 regarding the mean age ± SD from the whole population studied. C.A.B.A.: Ciudad Autónoma de Buenos Aires, the national capital city.
Table 2 Genotype and subtype assignment of the Argentinian isolates studied by using both 5'-untranslated region and NS5B sequences
HCV genotypeHCV[+] 5’UTR sequencesRelativeFrom total population (n = 6251)HCV subtypeHCV[+] NS5B sequencesRelativeFrom total population (n = 6251)
11155.00%0.176%1a525.00%0.080%
1b525.00%0.080%
115.00%0.016%
2735.00%0.112%2c525.00%0.080%
2j15.00%0.016%
215.00%0.016%
3210.00%0.032%3a15.00%0.016%
315.00%0.016%
Total20100.00%0.320%-17100.00%0.320%
1Untypeable at NS5B due to reverse transcription-heminested polymerase chain reaction amplification failure. HCV: Hepatitis C virus; 5’UTR: 5’-untranslated region.
Table 3 Genetic divergence (DNASP software)
Argentine general population sequencesGB deposited sequences from ArgentinaClosest GB deposited sequences
HCV-1a
Argentine general population sequences0.034
GB deposited sequences from Argentina0.0420.040
Closest GB deposited sequences0.0340.0370.032
HCV-1b
Argentine general population sequences0.082
GB deposited sequences from Argentina0.0740.046
Closest GB deposited sequences0.0540.0500.045
HCV-2c
Argentine general population sequences0.087
GenBank deposited sequences from Argentina0.0920.098
Closest GB deposited sequences0.0760.0800.076
Hepatitis C virus (HCV)-1b (Argentine general population sequences): 0.082 vs 0.054, P < 0.001; 0.082 vs 0.074, P > 0.05; HCV-1b [GenBank (GB) deposited sequences from Argentina]: 0.046 vs 0.050, P < 0.001; HCV-2c (Argentine general population sequences): 0.087 vs 0.076, P < 0.001; 0.087 vs 0.092, P > 0.05; HCV-2c (GB deposited sequences from Argentina): 0.098 vs 0.08, P < 0.001. All P (HCV-1a) > 0.05. Genetic divergence (DNASP software), considering three separate HCV groups: our Argentine general population (sequenced in this study), Argentinian sequences already deposited in GB, and the closest GB worldwide sequences. All values obtained were statistically paired and compared by using the χ2 test with Yates’ correction.
Table 4 Subtype assignment of each hepatitis C virus Argentinian sequence obtained in this study
IsolateNS5B genotypeClosest GB deposited sequencesOrigin
5329201aDQ8893001bosUnited States (Massachusetts, Boston area)
5335111aEU155310usa EU155290usa AY683093usa DQ8893001usaUnited States
P6061aAY682755alb AY683103alb AY682775alb DQ889318bos DQ889300bosUnited States (Albany, NY) United States (Massachusetts, Boston area)
T2941aDQ313467arg DQ313464arg DQ313465arg DQ313466arg DQ889300bosArgentina United States (Massachusetts, Boston area)
T2991aDQ889300bos DQ889296bos AY172640arg DQ889296bosUnited States (Massachusetts, Boston area) Argentina United States (Massachusetts, Boston area)
5336551bAF506602rus DQ345627madRussia (Western Siberia) Madagascar (Antananarivo)
5337711bDQ508484tun DQ508481tun DQ508479tun EF608896bcnTunisia (Tunis) Spain (Barcelona)
P2371bEU155224ten EU155304tenUnited States (Tennessee)
P8191bAY835061chn DQ249500usa DQ249499usa DQ345626madChina (Foshan) United States (factor VIII concentrate) Madagascar (Antananarivo)
P8991bDQ345626mad AJ132997ger AY682461alb AY683105alb DQ249501usaMadagascar (Antananarivo) Germany United States (Albany, NY) United States (factor VIII concentrate)
5324412cL38363swi D31981itaSwitzerland Italy (France from Italians)
5329962cL38365swi AF15995mar AF515996mar DQ220866touSwitzerland France (Marseille) France (Toulouse)
532978 5345882cD31975ita AY172649ita D50409(BEBE1)itaItaly (France from Italians)
T3002cDQ220870frFrance (Toulouse)
T3012cAF516025mar EF195026tall AY944641gen D31977itaFrance (Marseille) Estonia (Tallinn) Italy (Genoa) Italy (France from Italians)
5335702jAY89526que AY894529que AY894550que EF116050que DQ220919tou DQ220918tou D86530bcnCanada (Quebec) France (Toulouse) Spain (Barcelona)
T3023aEF116078que AJ291256ssd AJ867113arg AJ867159arg AJ867116arg AJ867115arg AJ867114argCanada (Quebec) France (Seine Saint Denis district) Argentina
The 3rd column from the left shows the GenBank (GB) sequences that are most similar to each of our samples, and the 4th column from the left exhibits the origin of the GB already deposited sequences.