Clonality analysis of neuroendocrine cells in gastric adenocarcinoma

Table 1 Primer sequences for the analysis of microsatellite instability and p53 mutations at exons 5-8

Microsatellite	Sequences
D1S104	ATCCTGCCCTTATGGAGTGCCCCACTCCTCTGTCATTGTA
D2S119	CTTGGGGAACAGAGGTCATTGAGAATCCCTCAATTTCTTTGGA
D2S123	AAACAGGATGCCTGCCTTTAGGACTTTCCACCTATGGGAC
D3S1766	ACCACATGAGCCAATTCTGTACCCAATTATGGTGTTGTTACC
D3S2427	CTCCTCGTCACTGCAGTCTTCTGCCTCATCTGTTCAGGAT
D4S174	AAGAACCATGCGATACGACTCATTCCTAGATGGGTAAAGC
D4S402	CTTACTGTGTTGCCCAAGGTAGCTCTATGATTCATTTCAAGTTTG
D5S107	GATCCACTTTAACCCAAATACGGCATCAACTTGAACAGCAT
D5S346	ACTCACTCTAGTGATAAATCGGGAGCAGATAAGACAGTATTACTAGTT
D5S409	GGGATGAAGTGTGGATAAACTAGGATGGCAGTGCTCTTAG
D7S1805	CCTGCTTTGGCTTACCTGTACCCACTTCTCTGCTATTACATAT
D9S157	AGCAAGGCAAGCCACATTTCTGGGGATGCCCAGATAACTATATC
D10S469	CAACAAGTGTGAGAGTCCATATGTTCTGTCTCTCCACAGT
AFMA086WG9	ATGTACGGTTCATTGACTTGACTGACTACAAATGGGCA
D11S861	CTGAAACCAAGTGAAAAGGAGAAAGCTCCATTGTCTTCTGGC
D12S1899	TTCTTCCTTTCTCTTTCTCTCTTCCGCACAAGTGACACATGGTCC
D16S398	CTTGCTCTTTCTAAACTCCAGAAACCAAGTGGGTTAGGTC
D16S496	GAAAGGCTACTTCATAGATGGCAATATAAGCCACTGCGCCCAT
D16S534	CAACAAAGCAAGACCCTGTCCATCTGCGGTTCTTTCCTC
D16S265	AGCTCTCTGAGTCCTCTGTGCGGAAGCATGGTGTCTCTCG
D16S752	AATTGACGGTATATCTATCTGTCTGGATTGGAGGAGGGTGATTCT
D17S250	GGAAGAATCAAATAGACAATGCTGGCCATATATATATTTAAACC
D17S796	CAATGGAACCAAATGTGGTCAGTCCGATAATGCCAGGATG
D19S416	CCTGTCCCAGAGAGACCCTAAAGAGAGTGTGCCATTTGCT
BAT 25	GTTTCGCCTCCAAGAATGTAAGTGTTTCTGCATTTTAACTATGGCTC
BAT 26	TGACTACTTTTGACTTCAGCCAACCATTCAACATTTTAACCC
Exon 5	GACTTTCAACTCTGTCTCCTCTGGGGACCCTGGGCAAC
Exon 6	GAGACGACAGGGCTGGGTCCACTGACAACCACCCTT
Exon 7	GTGTTGTCTCCTAGGTTGGCAAGTGGCTCCTGACCTGGAG
Exon 8	CCTTACTGCCTCTTGCTTTGAATCTGAGGCATAACTGC

Table 2 Concordance of p53 mutation in gastric cancer and neuroendocrine cells

Case	Cell	Exon	Codon	Mutation	Amino acid	Differentiation	TNM	Metastasis
4	Cancer	8	273	GC→AT	Arg→Cys	Poor	IV	+
	NE	8	273	GC→AT	Arg→Cys
7	Cancer	7	244	GC→AT	Gly→Ser	Poor	IV	+
	NE	8	287	GC→AT	Glu→Lys
14	Cancer	8	287	GC→AT	Glu→Lys	Poor	IB	+
	NE	8	287	GC→AT	Glu→Lys
21	Cancer	7	244	GC→AT	Gly→Ser	Poor	IV	+
	NE	7	244	GC→AT	Gly→Ser
27	Cancer	8	282	GC→AT	Arg→Arg	Moderate	III	+
	NE	8	282	GC→AT	Arg→Arg
17	Cancer	7	244	GC→AT	Gly→Ser	Poor	III	+

NE: Neuroendocrine; TNM: Tumour-node-metastasis.