Influence of up-regulation of Notch ligand DLL4 on biological behaviors of human gastric cancer cells

Table 1 Primer sequences for real-time polymerase chain reaction

Genes	Forward sequences	Reverse sequences	Product size (bp)
DLL4	5’-CCCTGGCAATGTACTTGTGAT-3’	5’-TGGTGGGTGCAGTAGTTGAG-3’	73
Notch1	5’-GCCTCAACATCCCCTACAAGA-3’	5’-CCACGAAGAACAGAAGCACAAA-3’	120
HES1	5’-GTCAACACGACACCGGATAA-3’	5’-TTCAGCTGGCTCAGACTTTC-3’	113
HES5	5’-TGGAGAAGGCCGACATCCT-3’	5’-GGCGACGAAGGCTTTGC-3’	65
HEY1	5’-CGCGTTATCTGAGCATCATT-3’	5’-TGGGAAGCGTAGTTGTTGAG-3’	88
MMP-2	5’-GCTGACGGTAAGGACGGACTC-3’	5’-CGTTGCCATTGAACAAGAAGG-3’	158
MMP-9	5’-TTTGACAGCGACAAGAAGTGG-3’	5’-AGGGCGAGGACCATAGAGG-3’	189
E-cadherin	CGTTAGAGGTGGGTGACTACAAA	GAACAGCAAGAGCAGCAGAAT	220
GAPDH	5’-TGCCACTCCTCCACCTTTG-3’	5’-CGAACCACCCTGTTGCTGT-3’	104

DLL: Delta-like ligand 4; MMP: Matrix metalloproteinases; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.