Dual probiotic strains suppress high fructose-induced metabolic syndrome

Table 1 Composition of high fructose diet

Ingredient	High fructose diet (g)
Casein	200.0
L-cystine	3.0
Fructose	700.0
Cellulose powder	50.0
Corn oil	25.0
Lard	20.0
Mineral Mix S10026	10.0
DiCalcium Phosphate	13.0
Calcium Carbonate	5.5
Potassium Citrate	16.5
Vitamin Mix V10001	10.0
Choline Bitartrate	2.0
Total	1000.0

Table 2 The composition of each supplement pack

	Placebo (mg)	109 probiotics (mg)	1010 probiotics (mg)
Lactobacillus curvatus HY7601	-	5.0 (5 × 108)	50.0 (5 × 109)
Lactobacillus plantarum KY1032	-	2.5 (5 × 108)	25.0 (5 × 109)
Lactose	100.0	92.5	25.
Total	100.0	100.0	100.0

Freeze-dried Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 concentration was 1 × 10⁸ and 2 × 10⁸ cfu/mg, respectively.

Table 3 Catalog numbers of Taqman probes

Taqman probe	Catalog number
Peroxisome proliferative activated receptor a	Rn00566193_m1
Carnitine palmitoyl transferase 1	Rn00580702_m1
Carnitine palmitoyl transferase 2	Rn00563995_m1
Acyl-CoA oxidase 1	Rn01460628_m1
Sterol regulatory element binding protein-1	Rn01495769_m1
Fatty acid synthase	Rn00569117_m1
Stearoyl-CoA desaturase 1	Rn00594894_g1
Cholesterol 7 alpha-hydroxylase	Rn00564065_m1
Low density lipoprotein receptor	Rn00598442_m1