Regulation of dipeptidyl peptidase 8 and 9 expression in activated lymphocytes and injured liver

doi:10.3748/wjg.v19.i19.2883

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May 21, 2013 (publication date) through Nov 29, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. May 21, 2013; 19(19): 2883-2893
Published online May 21, 2013. doi: 10.3748/wjg.v19.i19.2883

Table 1 Antibodies used in immunoblot and flow cytometry

Name	Isotype	Conjugate	Dilution	Supplier	Catalogue no.
Primary antibodies
CD4	Rat IgG_2b	FITC	1:50	BD Pharmingen, NJ, United States	557307
B220	Rat IgG	PE	1:50	Caltag Laboratories, CA, United States	RM2604-3
CD8	Rat IgG_2a	APC	1:50	BD Pharmingen, NJ, United States	553035
Annexin V		APC	1:50	BD Pharmingen, NJ, United States	550474
V5	Mouse monoclonal IgG_2a		1:5000	Invitrogen	R960-25
DPP8-catalytic domain	Rabbit polyclonal		1:2000	Abcam Inc	Ab42077
DPP8-catalytic domain	Rabbit polyclonal		1:2000	Abcam Inc.	Ab42076
DPP9-catalytic domain	Rabbit polyclonal		1:2000	Abcam Inc.	Ab42080
GAPDH	Mouse monoclonal		1:1000	EnCor biotechnology	MCA-1D4
β-actin	Rabbit polyclonal		1:1000	Sigma	A2103
Secondary antibodies
Anti-rabbit IgG	Goat	HRP	1:3000	DAKO, Carpinteria, CA, United States	PO448
Anti-mouse	Goat IgG	R-PE	1:400	Molecular Probes	P852

DPP: Dipeptidyl peptidase; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; IgG: Immunoglobulin G; FITC: Fluorescein; PE: Phycoerythrin; APC: Allophycocyanin; HRP: Horse radish peroxidase; R-PE: R-phycoerythrin.

Citation: Chowdhury S, Chen Y, Yao TW, Ajami K, Wang XM, Popov Y, Schuppan D, Bertolino P, McCaughan GW, Yu DM, Gorrell MD. Regulation of dipeptidyl peptidase 8 and 9 expression in activated lymphocytes and injured liver. World J Gastroenterol 2013; 19(19): 2883-2893
URL: https://www.wjgnet.com/1007-9327/full/v19/i19/2883.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i19.2883