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©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Feb 14, 2012; 18(6): 532-540
Published online Feb 14, 2012. doi: 10.3748/wjg.v18.i6.532
Published online Feb 14, 2012. doi: 10.3748/wjg.v18.i6.532
Table 1 List of primer sequences
| Primer | Forward primer (5'-3') | Reverse primer(5'-3') |
| RT-PCR | ||
| SFRP2 | 5’-GATGATGACAACGACATAATGGAAACG -3’ | 5’-GAGTGTGCTTGGGGAACGGGAGCT-3’ |
| GAPDH | 5’-CCCTTCATTGACCTCAACTACATGG-3’ | 5’-CATGGTGGTGAAGACGCCAG-3’ |
| SFRP2(CDS) | 5’-CCAAGCTTATGCTGCAGGGCCCTGGCTCGC-3’ | 5’-CGGAATTCCTAGCACTGCAGCTTGCGGATGCTG-3’ |
| MSP | ||
| SFRP2-M | 5’-GGGTCGGAGTTTTTCGGAGTTGCGC-3’ | 5’-CCGCTCTCTTCGCTAAATACGACTCG-3’ |
| SFRP2-U | 5’-TTTTGGGTTGGAGTTTTTTGGAGTTGTGT-3’ | 5’-AACCCACTCTCTTCACTAAATACAACTCA-3’ |
| BSP | ||
| SFRP2 | 5’-AAAAAGGTTAAGAAAATTTTGGT-3’ | 5’-AACCAAAACCCTACAACATC-3’ |
Table 2 Effect of secreted frizzled-related protein 2 re-expression on the cell cycle distribution of KYSE30 cells (%)
| Groups | G0/G1 | S | G2/M |
| KYSE-30/vector | 45.76 ± 3.74 | 48.64 ± 4.90 | 5.59 ± 5.18 |
| KYSE-30/SFRP2 | 64.49 ± 6.11 | 30.52 ± 5.07 | 4.97 ± 6.60 |
- Citation: Hao XW, Zhu ST, He YL, Li P, Wang YJ, Zhang ST. Epigenetic inactivation of secreted frizzled-related protein 2 in esophageal squamous cell carcinoma. World J Gastroenterol 2012; 18(6): 532-540
- URL: https://www.wjgnet.com/1007-9327/full/v18/i6/532.htm
- DOI: https://dx.doi.org/10.3748/wjg.v18.i6.532