Novel biosensor-based microarray assay for detecting rs8099917 and rs12979860 genotypes

Table 1 Sequence of primers and probes

Names	Sequences
IL28B primers forward (9860)	GTGCCTGTCGTGTACTGAAC
IL28B primers reverse (9860)	CGCTGAGCACTGCCTGGGC
IL28B primers forward (9917)	ATTTGTCACTGTTCCTCCT
IL28B primers reverse (9917)	GCCCTAACTGATACGCTATAA
rs12979860 probe (C allele)	AAAAAAAAAAAGCTCCCCGAAGGCGCGAACCAGGGTTGAAT
rs12979860 probe (T allele)	AAAAAAAAAAAGCTCCCCGAAGGCGTGAACCAGGGTTGAAT
rs8099917 probe (T allele)	AAAAAAAAAACTTTCTGTGAGCAATTTCACCCAAATTGGAA
rs8099917 probe (G allele)	AAAAAAAAAACTTTCTGTGAGCAATGTCACCCAAATTGGAA

IL28B: Interleukin 28B.

Table 2 Concordance of biosensor-based microarray assay with given plasmids for rs12979860 and rs8099917

BBM assay	Plasmids with given genotype			Total
	CC	CT	TT
rs12979860
CC	10	0	0	10
CT	0	10	0	10
TT	0	10	10	10
Total	10	10	10	30
rs8099917
TT	10	0	0	10
GT	0	10	0	10
GG	0	0	10	10
Total	10	10	10	30

Kappa = 1.00. BBM: Biosensor-based microarray.

Table 3 Concordance of biosensor-based microarray assay with polymerase chain reaction sequencing of the blood samples for rs12979860 and rs8099917

BBM assay	Direct sequencing			Total
	CC	CT	TT
rs129798601
CC	24	0	0	24
CT	3	11	0	14
TT	0	0	2	2
Total	27	11	2	40
rs80999172
TT	28	0	0	28
GT	2	9	0	11
GG	0	0	1	2
Total	30	9	1	40

¹Kappa = 0.88; ²Kappa = 0.85. BBM: Biosensor-based microarray.