Human papilloma virus 16 E6 oncoprotein associated with p53 inactivation in colorectal cancer

Table 1 Relationships between human papilloma virus 16 infection and clinical parameters of colorectal cancer patients n (%)

Parameters	HPV16
	Negative	Positive	P value
Gender			0.782
Male (n = 35)	29 (82.9)	6 (17.1)
Female (n = 34)	29 (85.3)	5 (14.7)
Age (yr)			0.322
< 68 (n = 31)	28 (90.3)	3 (9.7)
> 68 (n = 38)	30 (78.9)	8 (21.1)
Smoking status			0.718
No (n = 49)	42 (85.7)	7 (14.3)
Yes (n = 20)	16 (80.0)	4 (20.0)
Stage			0.017
I (n = 6)	2(33.3)	4 (66.7)
II (n = 24)	21 (87.5)	3 (12.5)
III (n = 21)	19 (90.5)	2 (9.5)
IV (n = 18)	16 (88.9)	2 (11.1)
Tumor site			0.554
Ascending colon (n = 22)	19 (86.4)	3 (13.6)
Transverse colon (n = 5)	5 (100.0)	0 (0)
Descending colon (n = 5)	3 (60.0)	2 (40.0)
Sigmoid colon (n = 10)	9 (90.0)	1 (10.0)
Rectum (n = 27)	22 (81.5)	5 (18.5)

HPV: Human papilloma virus.

Table 2 p53 mutation, p53 protein, E6 oncoprotein and clinical information in human papilloma virus 16 DNA-positive colorectal patients

PatientNo.	Gender	Age(yr)	Stage	Site	p53		HPV16
					Mutation	IHC	ISH	E6
101	F	65	I	d-colon	No	-	+	+
134	F	54	IIA	Rectum	No	+	+	+
141	M	72	I	Rectum	No	-	+	+
142	F	56	IIA	d-colon	Yes	++	+	+
167	M	70	IIIB	d-colon	Yes	+++	+	+
169	M	78	IV	Rectum	No	-	+	+
173	F	83	IIA	s-colon	Yes	+	+	+
200	M	74	IV	a-colon	Yes	+	+	+
226	F	78	I	Rectum	No	-	-	-
234	F	76	I	a-colon	No	+	-	-
259	F	79	IIIB	Rectum	Yes	++	-	-

HPV: Human papilloma virus; IHC: Immunohistochemistry; ISH: In situ hybridization. -: No positive staining; +: 1%-10%; ++: 1%-50%; +++: > 50% positive cells.

Table 3 p21 and Mdm2 mRNA expression levels in tumor tissues and adjacent normal tissues of E6-positive colorectal patients

Variable	E6-positive (n = 8)
	p53 wild-type (n = 4)	P	p53-mutation (n = 4)	P
P21 mRNA		0.025		0.061
Adjacent normal tissues	348.46 ± 143.89		241.12 ± 84.13
Tumor tissues	49.62 ± 16.58		122.96 ± 45.28
Mdm2 mRNA		0.032		0.043
Adjacent normal tissues	563.74 ± 252.28		541.50 ± 100.56
Tumor tissues	111.60 ± 75.99		317.74 ± 137.59