Lactobacillus crispatus M206119 exacerbates murine DSS-colitis by interfering with inflammatory responses

Table 1 Experimental protocol for induction of dextran sulfate sodium-colitis

Groups	Experimental design
Healthy control	No DSS treatments
Negative control (saline)	5% (w/v) DSS dissolved in drinking water for 7 d
	Saline administered intragastrically once daily for 9 d
Positive control (5-ASA)	5% (w/v) DSS dissolved in drinking water for 7 d
	5-ASA administered intragastrically once daily for 9 d
Investigating group (CCTCC M206119)	5% (w/v) DSS dissolved in drinking water for 7 d
	CCTCC M206119 administered intragastrically once daily for 9 d

DSS: Dextran sulfate sodium; 5-ASA: 5-aminosalicylic acid.

Table 2 Scoring of disease activity index

Score	Weight loss (%)	Stool consistency	Occult/gross fecal bleeding
0	0	Normal	Negative
1	1-5
2	5-10	Loose stool	Hemoccult
3	10-15
4	> 15	Diarrhea	Gross bleeding

DAI = (combined score of weight loss, stool consistency, and bleeding)/3. Normal stool, well-formed pellets; Loose stool, pasty and semi-formed stool that does not adhere to the anus; Diarrhea, liquid stool that adheres to the anus.

Table 3 Histological score to quantify the degree of colitis

Score	Inflammation	Depth of lesions	Destruction of crypt	Width of lesions (%)
0	None	None	None
1	Mild	Submucosa	1/3 basal crypt	1-25
2	Severe	Muscularis	2/3 basal crypt	26-50
3		Sera	Intact epithelium only	51-75
4			Total crypt and epithelium	76-100

Table 4 Primers used to detect gene expression at mRNA level

Genes		Sequence of primers
GAPDH	Sense	5’-ATCACCATCTTCCAGGAGCG-3’
	Anti-sense	5’-CCTGCTTCACCACCTTCTTG-3’
IL-1β	Sense	5’-TTTTAATCAGCTATCCGGAC-3’
	Anti-sense	5’-TAATGGGAACGTCACACACC-3’
IL-6	Sense	5’-ATGAAGTTCCTCTCTGCAAGAGACT3’
	Antisense	5’-CACTAGGTTTGTTTAATCTC-3’
TNF-α	Sense	5’-ACGTGGAACTGGCAGAAGAG-3’
	Antisense	5’-GGTTGTCTTTGAGATCCATGC-3’