Side-stream smoking reduces intestinal inflammation and increases expression of tight junction proteins

Table 1 Primer sets used for quantitative reverse transcriptase polymerase chain reaction of mouse large intestine tissue

Gene name	Accession no.	Product size	Direction	Sequence (5’→3’)	Source
IL-6	NM_031168.1	107 bp	Forward	GCTGGTGACAACCACGGCCT	This study
			Reverse	AGCCTCCGACTTGTGAAGTGGT
TNF-α	NM_013693.2	67 bp	Forward	TGGGACAGTGACCTGGACTGT	[58]
			Reverse	TTCGGAAAGCCCATTTGAGT
Claudin 3	NM_009902.4	132 bp	Forward	CAGGGGCAGTCTCTGTGCGAG	This study
			Reverse	GCCGCTGGACCTGGGAATCAAC
Occludin	NM_008756.2	308 bp	Forward	ATGTCCGGCCGATGCTCTC	[58]
			Reverse	TTTGGCTGCTCTTGGGTCTGTAT
ZO-1	NM_009386.2	403 bp	Forward	ACCCGAAACTGATGCTGTGGATAG	[58]
			Reverse	AAATGGCCGGGCAGAACTTGTGTA
ZO-2	AF113005.1	106 bp	Forward	CCCAGCACCAAGCCACCTTTTCA	This study
			Reverse	TCGGTTAGGGCAGACACACTCCC
GAPDH	NM_008084.2	132 bp	Forward	AACTTTGGCATTGTGGAAGG	This study
			Reverse	GGATGCAGGGATGATGTTCT

IL-6: Interleukin-6; TNF-α: Tumor necrosis factor-α; ZO: Zona occludens; GPADH: Glyceraldehyde-3-phosphate dehydrogenase.

Table 2 Primer sets used for quantitative polymerase chain reaction of 16S rRNA of specific bacterial species or genus

Target organism	Primer set	Sequence (5’ to 3’)	Product size	Annealing temp (°C)	Reference
Bacteroides	BactF285	GGTTCTGAGAGGAGGTCCC	53	61	[59]
	UniR338	GCTGCCTCCCGTAGGAGT		61
Clostridium butyricum	Cbut825F	GTGCCGCCGCTAACGCATTAAGTAT	213	72	[60]
	Cbut1038R	ACCATGCACCACCTGTCTTCCTGCC		72
Clostridium clostridiiforme	Cclos99F	AATCTTGATTGACTGAGTGGCGGAC	148	62	[60]
	Cclos247R	CCATCTCACACTACCGGAGTTTTTC		62
Clostridium perfringens	Cperf165F	CGCATAACGTTGAAAGATGG	104	61	[59]
	Cperf269R	CCTTGGTAGGCCGTTACCC		61
Enterobacteriaceae	Eco1457F	CATTGACGTTACCCGCAGAAGAAGC	195	63	[60]
	Eco1652R	CTCTACGAGACTCAAGCTTGC		63
Enterococcus	Ec-ssu1F	GGATAACACTTGGAAACAGG	115	60	[61]
	Ec-ssu1R	TCCTTGTTCTTCTCTAACAA		60
Eubacteria	UniF340	ACTCCTACGGGAGGCAGCAGT	210	63	[62]
	UniR514	ATTACCGCGGCTGCTGGC		63
Faecalibacterium prausnitzii	Fprau223F	GATGGCCTCGCGTCCGATTAG	199	58	[60]
	Fprau420R	CCGAAGACCTTCTTCCTCC		58
Lactoccoci	LabF362	AGCAGTAGGGAATCTTCCA	315	56	[59]
	LabR677	CACCGCTACACATGGAG		56
Mouse intestinal Bacteria	Uni516F	CCAGCAGCCGCGGTAATA	161	58	[59]
	MIBR677	CGCATTCCGCATACTTCTC		58
Segmented filamentous bacteria	SFB736F	GACGCTGAGGCATGAGAGCAT	108	58	[59]
	SFB844R	GACGGCACGGATTGTTATTCA		58
Ruminococcus albus	Ralb561F	CAGGTGTGAAATTTAGGGGC	246	63	[60]
	Ralb807R	GTCAGTCCCCCCACACCTAG		63