Transient micro-elastography: A novel non-invasive approach to measure liver stiffness in mice

Table 1 Characteristics of control mice and CCl₄-induced fibrotic mice

Mouse	Strain	Type	Sex	Liver stiffness (kPa)	Success rate for LSM (%)	% sirius red
1	C57BL/6	Control	M	4.7	75	ND
2	C57BL/6	Control	M	4.0	100	ND
3	C57BL/6	Control	M	4.7	67	ND
4	C57BL/6	Control	M	6.8	86	ND
5	CD1	Control	F	5.0	100	< 0.3
6	CD1	Control	F	3.3	92	0.05
7	CD1	Control	F	2.6	94	0.10
8	CD1	Oil injected	F	5.3	100	0.42
9	CD1	Oil injected	F	9.3	84	0.72
10	CD1	Oil injected	F	5.8	91	0.32
11	CD1	CCl4 injected	F	18.8	100	1.84
12	CD1	CCl4 injected	F	21.6	100	2.36
14	CD1	CCl4 injected	F	14.2	100	2.38

LSM:Liver stiffness measurement ; ND: Not determined.

Table 2 Characteristics of the amyloidosis group mice

Mouse	Strain	Sex	Liver weight to body weight ratio (%)	hapoA-II (g/L)	Amyloidosis index	LS (kPa)	Success rate for LSM (%)
1	Y	F	5.1	0.19	0	3.9	90
2	Y	M	ND	ND	0	9.3	83
3	Y	F	ND	ND	0	5.9	100
4	Y	F	4.4	0.22	0	4.1	100
5	Y	F	4.5	0.22	0	4.1	100
6	Y	F	4.6	ND	0	4.7	86
7	Y	F	ND	ND	1	12.6	100
8	Y	M	5.2	0.23	0	3.9	100
9	Y	F	ND	ND	0	11.2	92
10	K	M	ND	0.15	1	23.7	100
11	K	M	16.0	0.55	4	124.0	74
12	K	M	13.8	0.57	2	124.0	57
13	K	F	15.8	0.54	4	168.8	100
14	K	F	22.8	0.80	3	168.8	82
15	K	F	18.2	0.44	3	168.8	100
16	K	F	12.1	0.42	2	94.9	100
17	K	F	5.0	0.28	0	4.4	96
18	F	M	7.6	0.66	1	50.2	100
19	F	M	ND	0.76	1	73.0	100
20	F	M	ND	0.44	1	13.5	92
21	F	F	12.9	ND	2	124.0	95
22	F	M	ND	0.35	2	155.5	100
23	F	F	16.3	0.58	3	168.8	95
24	F	M	15.7	0.53	3	168.8	69
25	F	M	14.9	0.59	3	168.8	100
26	F	M	13.7	0.64	3	124.0	89
27	F	M	10.8	0.59	3	168.8	100

LSM: Liver stiffness measurement; ND: Not determined.

Table 3 Macroscopic liver amyloidosis index obtained by visual assessment and manual palpation

Index	Liver appearance
0	Normal
1	Slightly bigger and/or stiffer
2	Big and stiff (± whitish zones)
3	Very big and stiff, changes in the right lobe (± whitish zones)
4	Very big and stiff, right lobe transformed and very big (± whitish zones)