IκB kinase-beta inhibitor attenuates hepatic fibrosis in mice

Table 1 Pathological scores in liver tissues

Groups	P + L + 2 × (PN + BN)	S0 S1 S2 S3 S4 Z
Control	0	5 0 0 0 0
HF	10.70 ± 2.62a	0 3 2 0 0
HF + LPS	13.30 ± 3.83a	0 1 3 1 0
HF + LPS + IMD	4.60 ± 3.83ac	0 4 1 0 0 -2.35e

The score of inflammation is given by P + L + 2 × (PN + BN).

^aP < 0.01 vs the control group;

^cP < 0.01 vs the HF and HF + LPS groups;

^eZ = -2.35, P = 0.018, P < 0.05 vs the HF + LPS group. P: Portal area inflammation; L: Lobular inflammation; PN: Patch necrosis; BN: Bridging necrosis; HF: High-fat; LPS: Lipopolysaccharide; IMD: IKK2 inhibitor.

Table 2 Oligonucleotide sequences used in real-time polymerase chain reaction

mRNA	Sequence	Length (bp)
TypeI(Iα) collagen	F: ACAGTGGTGAACCTGGTGCT	151
	R: CTCCTTTGGCACCAGTGTCT
Type III collagen	F: GGAGCCCCTGGACTAATAG	193
	R: ATCCATCTTTGCCATCTTCG
α–SMA	F: TGCTGTCCCTCTATGCCTCT	185
	R: GAAGGAATAGCCACGTCAG
TGF-β1	F: CTTGCCCTCTACAACCAACA	189
	R: CTTGCGACCCACGTAGTAGA
β-actin	F: TGTGTCCGTCGTGGATCTGA	126
	R: CTTGCGACCCACGTAGTAGA

α–SMA: Alpha-smooth muscle actin; TGF-β1: Tumor growth factor-beta1.