Dickkopf3 overexpression inhibits pancreatic cancer cell growth in vitro

doi:10.3748/wjg.v17.i33.3810

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Sep 7, 2011 (publication date) through Oct 22, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Sep 7, 2011; 17(33): 3810-3817
Published online Sep 7, 2011. doi: 10.3748/wjg.v17.i33.3810

Table 1 Oligonucleotide primers used in the study

	Sequence (5’ to 3’)	T_A (°C)	Cycles
PCR
Dkk3 (full-length)	Forward: CCCAAGCTTATGCAGCGGCTTGGGGC	53	35
Dkk3 (full-length)	Reverse: CGCGGATCCCTAAATCTCTTCCCCTCCCAGCAGT	53	35
Real-time RT-PCR
Dkk3	Forward: ACAGCCACAGCCTGGTGTA	60	40
Dkk3	Reverse: CCTCCATGAAGCTGCCAAC	60	40
β-catenin	Forward: AAAATGGCAGTGCGTTTAG	60	40
β-catenin	Reverse: TTTGAAGGCAGTCTGTCGTA	60	40
GAPDH	Forward: GCACCGTCAAGGCTGAGAAC	60	40
GAPDH	Reverse: GCCTTCTCCATGGTGGTGAA	60	40
RT-PCR
Dkk3	Forward: AAGGCAGAAGGAGCCACGAGTGC	61	35
Dkk3	Reverse: GGCCATTTTGGTGCAGTGACCCCA	61	35
β-actin	Forward: AAATCGTGCGTGACATTAA	52	35
β-actin	Reverse: CTCGTCATACTCCTGCTTG	52	35
MSP
Dkk3 unmethylated	Forward: TTAGGGGTGGGTGGTGGGGT[32]	59	34
Dkk3 unmethylated	Reverse: CTACATCTCCACTCTACACCCA[32]	59	34
Dkk3 methylated	Forward: GGGCGGGCGGCGGGGC[32]	59	34
Dkk3 methylated	Reverse: ACATCTCCGCTCTACGCCCG[32]	59	34

T_A: Annealing temperature; Real-time RT-PCR: Real-time reverse transcription polymerase chain reaction; Dkk3: Dickkopf3; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; MSP: Methylation-specific polymerase chain reaction.

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Citation: Gu YM, Ma YH, Zhao WG, Chen J. Dickkopf3 overexpression inhibits pancreatic cancer cell growth in vitro. World J Gastroenterol 2011; 17(33): 3810-3817
URL: https://www.wjgnet.com/1007-9327/full/v17/i33/3810.htm
DOI: https://dx.doi.org/10.3748/wjg.v17.i33.3810