Protective effects of ACLF sera on metabolic functions and proliferation of hepatocytes co-cultured with bone marrow MSCs in vitro

Table 1 Biochemical parameters in plasma samples of 18 patients with acute-on-chronic liver failure

Case No.	Tbil (μ mol/L)	Dbil (μ mol/L)	ALB (g/L)	ALT (U/L)	AST (U/L)	CHE (U/L)	ALP (U/L)	LDH (U/L)	γ-GT (U/L)
1	306	168	34	218	342	3449	152	218	69
2	489	278	32	96	113	2896	136	288	74
3	456	281	36	202	315	1813	223	278	86
4	889	483	39	156	155	2224	195	279	49
5	400	202	33	65	104	3002	170	202	34
6	342	179	31	184	237	2408	138	197	42
7	456	263	33	261	158	1565	148	222	58
8	324	205	28	139	178	2672	125	234	36
9	591	366	32	87	101	2606	189	246	96
10	569	286	38	93	67	4737	121	266	104
11	425	268	43	187	108	5732	131	138	109
12	396	199	32	56	138	2783	138	277	38
13	499	313	34	29	98	4157	294	288	47
14	558	298	31	412	219	2025	104	245	99
15	239	151	28	235	236	1655	136	452	189
16	199	103	40	2343	2773	3858	147	1154	173
17	319	167	33	144	153	1698	116	276	93
18	301	222	33	64	95	3317	99	150	111

ALB: Albumin; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; ALP: Alkaline phosphatase; CHE: Concentrations of acetylcholine; LDH: Lactate dehydrogenase.

Table 2 Chemokine profiles in liver failure serum vs normal serum

	NS group (n = 18)	FS group (n=18)	P value
HGF (pg/mL)	469.47 ± 134.87	5253.70 ± 1876.35	0.002
EGF (pg/mL)	282.56 ± 41.55	74.63 ± 18.36	0
VEGF (pg/mL)	99.27 ± 19.42	ND	0
TNF-α	66.48 ± 28.56	168.86 ± 38.69	0.001

NS: Normal serum; FS: Liver failure serum; ND: Undetectable; HGF: Hepatocyte growth factor; EGF: Epidermal growth factor; VEGF: Vascular endothelial growth factor; TNF-α: Tumor necrosis factor-alpha.

Table 3 Hepatocyte ratio of G2-S phase in Hep-F group and Co-F group

	Concentration of serum %
	10	20	40	60	80	100
G2-S phase in Hep-F group	9.26	13.47	18.66	23.43	13.99	7.81
G2-S phase in Co-F group	16.02	23.28	28.25	32.08	31.24	9.94

Hep-F: Homo-hepatocytes cultured by various concentrations of liver failure serum; Co-F: Albumin in the medium of both liver failure serum; G2-S: Gap 2-Synthesis.