CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma

Table 1 Primers and fluorescent probe for human CXC chemokine receptor-4, stromal cell-derived factor-1 and GAPDH

Primers	5'-3'	Product (bp)
CXCR4	Forward: TGGCCTTATCCTGCCTGGTAT	173
	Reverse: GGAGTCGATGCTGATCCCAAT
	Taqman: AGAAGCGCAAGGCCCTCAAGACCA
SDF-1	Forward: GAGCCAACGTCAAGCATCTCA	103
	Reverse: TTCGGGTCAATGCACACTTGT
	Taqman: CTGTGCCCTTCAGATTGTAGCCCGG
GAPDH	Forward: TCATGGGTGTGAACCATGAGAA	146
	Reverse: GGCATGGACTGTGGTCATGAG
	Taqman: TCATCAGCAATGCCTCCTGCACCA

CXCR4: CXC chemokine receptor-4; SDF-1: Stromal cell-derived factor-1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Table 2 Expression of CXC chemokine receptor-4 in primary gastric carcinoma and normal mucous membrane (mean ± SE)

Tissues	No.	CXCR4-mRNA	t	P
Gastric cancer	40	1.6244 ± 1.3801	2.554	0.015
Normal mucous membrane	40	1.0715 ± 0.5243

CXCR4: CXC chemokine receptor-4.

Table 3 Expression of CXC chemokine receptor-4 in primary gastric carcinoma with or without lymph nodes metastasis (mean ± SE)

Lymph node metastasis	No.	CXCR4-mRNA	t	P
Present	32	0.823 ± 0.551	2.101	0.042
Absent	8	0.392 ± 0.338

CXCR4: CXC chemokine receptor-4.

Table 4 Relationship between CXC chemokine receptor-4 expression and clinicopathological features in gastric cancer

Clinicopathologic parameters	No.	CXC chemokine receptor-4
		Positive	Negative	χ2	P value
Sex
Male	31	18	13	0.006	0.9382
Female	9	6	3
Age (yr)
> 56	19	12	7	0.007	0.9351
≤ 56	21	13	8
Tumor size (cm)
≥ 5	25	16	9	0.064	0.8001
< 5	15	9	6
Tumor location
Cardia of stomach	2	2	0	-	0.8113
Fundus of stomach	1	1	0
Body of stomach	6	4	2
Antrum of stomach	31	18	13
Differentiation
Moderate/well	6	1	5	-	0.0213
Poor	34	24	10
Lymph node metastasis
Present	32	26	6	4.146	0.0422
Absent	8	3	5
Stage
II and IIIa	23	8	15	5.013	0.0251
IIIb and IV	17	12	5

¹χ² test;

²Modified χ² test;

³Fisher exact test.

Table 5 Expression of stromal cell-derived factor-1 in lymph nodes with or without gastric cancer cell metastasis (mean ± SE)

Lymph node metastasis	No.	SDF-1-mRNA	t	P
With	24	0.5432 ± 0.4907	2.338	0.025
Without	16	0.2640 ± 0.2601

SDF-1: Stromal cell-derived factor-1.