Proteomic analysis of pancreatic intraepithelial neoplasia and pancreatic carcinoma in rat models

Table 1 Sample markers and gel distribution in 2D-DIGE analysis

Gel	Cy2	Cy3	Cy5
Gel1	Internal standard	A1	B3
Gel2	Internal standard	B3	C2
Gel3	Internal standard	C1	A2
Gel4	Internal standard	B2	C3
Gel5	Internal standard	A3	B1

Table 2 Differentially expressed proteins identified by matrix-assisted laser desorption/ionization-time of flight/time of flight tandem mass spectrometry in normal pancreatic, pancreatic intraepithelial neoplasia-2 and pancreatic cancer tissues

Spot position	Protein number in NCBI	Protein name	MW	PI	Mascot PMF score	P
230	Mixture,gi|6753406 + gi|74205924	Carboxyl ester lipase+unnamed protein product	70	6	136	4.10E-5
780	gi|158186649	Enolase 1	40	5.5	76	5.40E-5
1205	gi|6678437	Tumor protein translationally controlled 1	25	5	98	7.90E-5
1402	gi|55926145	Expressed in non-metastatic cells 2	20	8	185	0.000
1132	gi|149024340	Pancreatic elastase 3B	25	5.5	91	0.000
1066	gi|56676354	Necdin	30	8	63	0.000
1272	gi|6435547	Hbp23	25	7.5	62	0.001
226	gi|6753406	Carboxyl ester lipase	90	5.5	118	0.001
1405	gi|109488364	Chromodomain helicase DNA-binding protein 3	20	5.5	72	0.001
755	Mixture,gi|149033753 + gi|149039895	Albumin+retinoid X receptor-interacting protein	40	6.5	181	0.001
1259	gi|12832572	Unnamed protein product	25	6	38	0.001
298	gi|149063967	Phosphoenolpyruvate carboxykinase 2	66	7.5	229	0.002
1057	gi|4504447	Heterogeneous nuclear ribonucleoprotein A2/B1	30	8.5	62	0.002
993	gi|5174447	Guanine nucleotide binding-protein (G-protein), beta polypeptide 2-like 1	30	7.5	146	0.003
1040	gi|5174447	Guanine nucleotide binding-protein (G-protein), beta polypeptide 2-like 1	30	5.5	142	0.004
658	gi|66730435	Glycine C-acetyltransferase	40	8	211	0.008

NCBI:National Center for Biotechnology Information; PMF:Peptide mass fingerprint; MW: Molecular weight ; PI: Isoelectric point.