Braun S, Berg C, Buck S, Gregor M, Klein R. Catalytic domain of PDC-E2 contains epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis. World J Gastroenterol 2010; 16(8): 973-981 [PMID: 20180236 DOI: 10.3748/wjg.v16.i8.973]
Corresponding Author of This Article
Reinhild Klein, MD, Associate Professor, Department of Internal Medicine II, University of Tuebingen, Otfried-Muller-Str. 10, 72076 Tuebingen, Germany. reinhild.klein@med.uni-tuebingen.de
Article-Type of This Article
Brief Article
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Table 3 Reactivity and incidence of IgM-antibodies to 33 PDC-E2 peptides in PBC patients as compared to healthy individuals n (%)
Peptide No.
Amino acid
PBC patients
Healthy individuals (n = 22)
Anti-M2+ (n = 95)
AMA-/ANA+ (n = 11)
Outer lipoyl domain (aa 1-80)
1
-2-23
7 (7)
0
1 (5)
2
16-40
10 (11)
0
2 (9)
3
33-57
27 (28)
0
0
4
50-74
33 (35)
1 (9)
1 (5)
5
67-91
42 (44)
0
1 (5)
Hinge
6
84-108
31 (33)
0
1 (5)
7
101-125
52 (55)1
1 (9)
2 (9)
Inner lipoyl domain (aa 133-217)
8
118-142
34 (36)
0
1 (5)
9
135-159
35 (37)
0
2 (9)
10
152-176
35 (37)
0
1 (5)
11
169-193
37 (39)2
0
2 (9)
12
186-210
27 (28)
0
1 (5)
13
203-227
23 (24)
0
2 (9)
Hinge
14
220-244
26 (27)
0
0
15
237-261
43 (45)
0
3 (14)
16
254-278
33 (35)
0
1 (5)
E1/E3-binding site (aa 272-303)
17
271-295
15 (16)
0
0
18
288-312
24 (25)
0
1 (5)
Hinge
19
305-329
36 (38)
0
3 (14)
Catalytic site (aa 331-561)
20
322-346
1 (1)
0
0
21
339-363
4 (4)
0
0
22
356-380
24 (25)
0
1 (5)
23
373-397
10 (11)
0
0
24
390-414
23 (24)
1 (9)
3 (14)
25
407-431
55 (58)3
0
1 (5)
26
424-448
21 (22)
0
0
27
441-465
16 (17)
0
0
28
458-482
15 (16)
0
0
29
475-499
70 (74)4
0
1 (5)
30
492-516
14 (15)
0
2 (9)
31
509-533
6 (6)
0
2 (9)
32
526-550
4 (4)
0
0
33
543-561
9 (9)
0
1 (5)
Table 4 Reactivity of sera from 95 anti-M2 positive PBC patients with different peptide conjugates containing the reported immunodominant epitope of PDC-E2[6,12]
Citation: Braun S, Berg C, Buck S, Gregor M, Klein R. Catalytic domain of PDC-E2 contains epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis. World J Gastroenterol 2010; 16(8): 973-981