Primary mixed germ cell tumor of the liver with sarcomatous components

Table 1 Immunohistochemical and FISH profiles of tumor that is considered in the differential diagnosis of GCT of the liver

Markers	Yolk sac tumor component	Immature glandular structure	Primitive mesenchymal component	Rhabdomyoblastic component	Chondroid and osteoblastic components	Neuroblastoma component
Antigen
AFP	+++	+1	-	-	+	-
AE1/AE3	+++	+++	-	-	-	-
Hep par 1	-	+	-	-	-	-
CK7	-	+	-	-	-	-
CK18	+	+++	-	-	-	-
CK19	+++	+++	-	-	-	-
CK20	-	+	-	-	-	-
EMA	-	+	-	-	-	-
α-1-antitrypsin	+	+	-	-	-	-
α-1-chymotrypsin	-	+	-	-	-	-
pCEA	-	++	-	-	-	-
mCEA	-	++	-	-	-	-
Vimentin	-	-	+++	+++	++	+++
Desmin	-	-	-	+++	-	-
Myoglobin	-	-	-	+++	-	-
SMA	-	-	+1	-	-	-
CD30	-	-	-	-	-	-
HCG	-	-	-	-	-	-
GFAP	+	-	-	-	-	++
NSE	-	-	++	-	-	+++
S-100	-	-	-	-	++	++
Synaptophysin	-	-	-	-	-	++
PLAP	-	-	-	-	-	-
CD56	-	-	+	-	-	++
HMB45	-	-	-	-	-	+1
CD117	-	-	++	-	-	-
CD34	-	-	+1	-	-	-
Ki67	+++	+++	+	++	-	+++
P53	+++	+++	-	++	-	++
CD45	-	-	-	-	-	-
FISH	+	+	-	+	-	-

¹Focal. +: Positive; ++: Strongly positive; -: Negative; FISH: Fluorescence in situ hybridization; GCT: Germ cell tumor; AFP: α-fetoprotein; EMA: Epithelial membrane antigen; CEA: Carcinoembryonic antigen; SMA: Smooth muscle actin; HCG: Human chorionic gonadotropin; GFAP: Glial fibrillary acid protein; NSE: Neuron-specific enolase; PLAP: Placental alkaline phosphatase.