Brief Article
Copyright ©2010 Baishideng Publishing Group Co.
World J Gastroenterol. Oct 28, 2010; 16(40): 5092-5103
Published online Oct 28, 2010. doi: 10.3748/wjg.v16.i40.5092
Table 1 Primer sets used for reverse transcription-polymerase chain reaction analysis
GeneForward primer Reverse primerProduct size (bp)
AlbTGCTTGAATGTGCTGATGACAGGGAAGGCAAGTCAGGGCATCTCATC161
AFPTGCAGCCAAAGTGAAGAGGGAAGACATAGCGAGCAGCCCAAAGAAGAA216
TATTGAGCAGTCTGTCCACTGCCTATGTGAATGAGGAGGATCTGAG358
G6PGCTGGAGTCCTGTCAGGCATTGCTAGAGCTGAGGCGGAATGGGAG350
TOATACAGAGACTTCAGGGAGCTGGTTGGGTTCATCTTCGGTATC299
HNF-1αGTGTCTACAACTGGTTTGCCTGTAGACACTGTCACTAAGG251
HNF-3βCACCACTACGCCTTCAACCGGTAGTAGGAGGTATCTGCGG235
HNF-4CTGCTCGGAGCCACAAAGAGATCCATGATCATCTGCCACGTGATGCTCTGCA371
C/EBPαCAAGAAGTCGGTGGACAAGAACCCTCATCTTAGACGCACCAAGT450
β2MTGACGTGTGAACCATGTGGAGACAGCACTCAAAG242
Table 2 Primer sets for real-time reverse transcription-polymerase chain reaction analysis
GeneForward primerReverse primerProbe
HNF-1αTGAGTCCGGGCTTCACACGGCTGCTGGAGGACACTG42
HNF-3βGCACCTGCAGATTCTGATTTTGACTTCCCTGCAACAACAGC25
HNF-4αATTGACAACCTGTTGCAGGACGTTGGTTCCCATATGTTCC77
HNF-4γCTTGGTGGAATGGGCTAAAAGCTCTCAACAGTGCCACCT8
HNF-6CCTGGAGCAAACTCAAATCCTTCTTTCCTTTTGCATGCTG88
C/EBPαCAACACTTGTATCTGGCCTCTGCGAGCAAAACCAAAACAAAAC3
PPARαGTCCCCGCAGATTCTACATTGAAGCTGTCCTGGCTCAGAT14
AlbTGTTGCCAAGCTGCTGATACCTTCATCCCGAAGTTCATC27
TATTGCTGAGCAGTCTATCCACTCTGCTCACAGAACTCCTGGAT67
G6PGCTGCTCATTTTCCTCATCAATTCTGTAACAGCAATGCCTGA67
CYP1A1GACAGATCCCATCTGCCCTACAAACTTGTGTCTCTTGTTGTGC80
CYP1A2CAAGAAATGCTGTGTCTTCGTAAAGAGGGGTCCTCCCACAG59
CYP2A6CAAAAAGGACACCAAGTTTCGAGAGCCCAGCATAGGGTACA69
CYP2B6GAAGCTTTTATCCCCTTCTCCTGCCATGGAGAAGTTCTGGAG35
CYP2C8AAGAAAAGTGACTACTTCATGCCTTTCAAGTCCTTCTCCTGCACAA18
CYP2C9ATTGACCTTCTCCCCACCACAGGGAAATTAATATGGTTGTGC43
CYP2C19GTCCAGAGATACATCGACCTCAAGTGAGGGAAGTTAATATGGTTGTG43
CYP2D6AGGAGGAGTCGGGCTTTCTCGCTGGGATATGCAGGAG56
CYP2E1CAAGCCATTTTCCACAGGACAACAAAAGAAACAACTCCATGC67
HPRTAGGTTGCAGCGAGGAAAAAAGCCACCCTGGAAGAAACTT59