Adhesion and immunomodulatory effects of Bifidobacterium lactis HN019 on intestinal epithelial cells INT-407

Table 1 Primer sequences of genus-specific genes used in adhesion assays

Primer name	Primer sequences (5’-3’)	Specificity	Ref.
BIF 164	GGGTGGTAATGCCGGATG	Bifidobacterium	[37]
BIF 662	CCACCGTTACACCGGGAA
MINf	ACGGTAACAGGAAGCAG	Salmonella	[38]
MINr	TATTAACCACAACACCT

Table 2 Primer sequences used for quantitative real-time PCR analysis

Gene	Primer sequences (5’-3’)	Product size (bp)
IL-1β	ACTCACTTAAAGCCCGCCTG	80
	TCAGAATGTGGGAGCGAATG
IL-8	CAAGAGCCAGGAAGAAACCAC	101
	TGCAGAAATCAGGAAGGCTG
TNF-α	TCTTCTCGAACCCCGAGTGA	150
	CCTCTGATGGCACCACCAG
β-actin	TGTTACAGGAAGTCCCTTGCC	101
	AATGCTATCACCTCCCCTGTG

PCR: Polymerase chain reaction; IL: Interleukin; TNF: Tumor necrosis factor.