Aspirin increases susceptibility of Helicobacter pylori to metronidazole by augmenting endocellular concentrations of antimicrobials

Table 1 Primers and probes used in real-time quantitative PCR

Probe or primer type	Sequence (5'-3')
hopA F	ATCATGCTAGTGATGGCGTTAAAG
hopA R	CAGGCATAGACGGAGGCAAT
hopA Probe	CCAAAAATATTGCATGCGTTCCCGC
hopB F	CTTGGTGCAAAACATCGTCAAT
hopB R	CCCGCCATAGCTCACTTGAT
hopB Probe	TAACGCTAGCCAACAACGCTAACATCAGCA
hopC F	CGCTCTTTATAACGCGCAAGTAA
hopC R	GCTGTTCCCGGCTCTGAAT
hopC Probe	TGGATAAAATCAACGCGCTCAACAATCAG
hopD F	CTGCTTGAGCGCGGTTTAA
hopD R	CAACCTAGACACTGGGAAAGCAT
hopD Probe	CTTGCGCTCTAGCGTTAGCGAACATGC
hopE F	GGATTGCACAGGGAGTGTTGT
hopE R	GCCCCATTAGCGTATTTAGCAT
hopE Probe	TTGCCCCCCAGGTCTTACCGCT
hefA F	AGGGCGTTTTGGGAATTTCT
hefA R	GCATGATGGATTGTTTTTGCA
hefA Probe	CCCCGGTCAGCAAAATACGGCTG
hefB F	AGGGCGATGTTTTGTTGCTT
hefB I	CCCCCAATTTTGCTGTATCGT
hefB Probe	AATCAAGACAAACAGGCTCAAAGCGATTCC
hefC F	GTTTGCGTTCTTGCGTAACG
hefC R	TGTTTAATGAAAAGCCCATCCA
hefC Probe	CACGATCACCCTCGTTTCAGCGATC
16S rRNA F	CCGCCTACGCGCTCTTTAC
16S rRNA R	CTAACGAATAAGCACCGGCTAAC
16S rRNA Probe	CCCAGTGATTCCGAGTAACGCTTGCA

Table 2 cDNAs levels of porins and efflux system of H pylori tested by real-time quantitative PCR

	Levels of cDNA
Gene	Aspirin group	DMSO control	Blank control
hopA	963.60 ± 23.33	1087.75 ± 26.42	856.27 ± 25.43
hopB	84.00 ± 15.97	91.45 ± 14.83	100.15 ± 16.28
hopC	52.98 ± 11.67	51.77 ± 12.01	52.79 ± 10.27
hopD	83.86 ± 14.09	92.46 ± 17.78	89.92 ± 13.72
hopE	54.07 ± 10.58	52.77 ± 11.93	52.12 ± 9.92
hefA	12.31 ± 2.12	12.51 ± 1.99	12.97 ± 2.76
hefB	25.47 ± 4.73	28.48 ± 5.07	25.60 ± 4.99
hefC	55.62 ± 8.89	52.08 ± 9.78	53.37 ± 10.04

P > 0.05 (Aspirin group compared with DMSO control or blank control).