N-cadherin knock-down decreases invasiveness of esophageal squamous cell carcinoma in vitro

Table 1 Real-time PCR primers for N-cadherin, E-cadherin, MMP-9 and GAPDH

Target gene	Primers	Length of product (bp)
N-cadherin	Sense: 5’-GGTGGAGGAGAAGAAGACCAG-3’	72
	Antisense: 5’-GGCATCAGGCTCCACAGT-3’
E-cadherin	Sense: 5’-CCCGGGACAACGTTTATTAC-3’	72
	Antisense: 5’-GCTGGCTCAAGTCAAAGTCC-3’
MMP9	Sense: 5’-GAACCAATCTCACCGACAGG-3’	67
	Antisense: 5’-GCCACCCGAGTGTAACCATA-3’
GAPDH	Sense: 5’-AGCCACATCGCTCAGACA-3’	66
	Antisense: 5’-GCCCAATACGACCAAATCC-3’

Table 2 Correlations of E-cadherin, N-cadherin expression with clinicopathological features of ESCC

Items	n	E-cadherin			N-cadherin
		Cases (%)	χ2	P	Cases (%)	χ2	P
Histological classification
NEE	62	59 (95.2)			18 (29.0)
AH	31	22 (71.0)	48.426	0.000	19 (61.3)	29.091	0.000
ESCC	62	25 (40.3)			47 (75.8)
Histological grade
I	15	11 (73.3)			8 (53.3)
II	25	9 (36.0)	9.962	0.007	19 (76.0)	6.924	0.031
III	22	5 (22.7)			20 (90.9)
Depth of invasion
Not to serosa	7	6 (85.7)	4.797	0.029	2 (28.6)	6.916	0.009
To serosa	55	19 (34.5)			45 (81.8)
Lymph node metastasis
Yes	42	21 (50.0)	3.897	0.048	28 (66.7)	4.486	0.034
No	20	4 (20.0)			19 (95.0)

NEE: Normal esophageal squamous tissues; AH: Adjacent atypical hyperplastic epithelium; ESCC: Esophageal squamous cell carcinoma tissues.

Table 3 Correlation between E-cadherin and N-cadherin protein expressions

	E-cadherin	N-cadherin (+)	N-cadherin (-)	γ	P
+	25	12	13	-0.534	0.000
-	37	35	2