©2009 The WJG Press and Baishideng.
World J Gastroenterol. Nov 28, 2009; 15(44): 5610-5619
Published online Nov 28, 2009. doi: 10.3748/wjg.15.5610
Published online Nov 28, 2009. doi: 10.3748/wjg.15.5610
Table 1 The position, polymorphism and primer sequence in IL-2, IL-4 and IL-10 genes
| SNP position | Polymorphism | Primer sequence 5’-3’ | PCR conditions |
| IL-2 -330 | T→G | F: 5'-TATTCACATGTTCAGTGTAGTTCT-3' | 94°C, 48°C, 72°C for 1 min |
| R: 5'-ACATTAGCCCACACTTAGGT-3' | |||
| IL-4 -589 | C→T | F: 5'-ACTAGGCCTCACCTGATACG-3' | 94°C, 57°C, 72°C for 30 s |
| R: 5'-GTTGTAATGCAGTCCTCCTG-3' | |||
| IL-10 -592 | A→C | F: 5'-CCTAGGTCACAGTGACGTGG-3' | 94°C/30 s, 60°C/45 s, 72°C/60 s |
| R: 5'-GGTGAGCACTACCTGACTAGC-3' |
Table 2 Polymorphism position, product size, restriction enzyme, digestion fragments in IL-2-330, IL-4-589 and IL-10-592
| SNP position | Product size (bp) | Restriction enzyme | Digestion fragments (bp) |
| IL-2-330 T/G | 150 | MaeI (Bio Basic Inc.) | 150 |
| 26 + 124 | |||
| IL-4-589 T/C | 252 | BsmFI (New England BioLabs) | 252 |
| 60 + 192 | |||
| IL-10-592 C/A | 412 | RsaI (Bio Basic Inc.) | 412 |
| 236 + 176 |
Table 3 HWE test for cytokines
| Locus | Observed homozygosity (%) | Expected homozygosity (%) | P value | ||
| Wild-type genotype | Mutant genotype | Wild-type genotype | Mutant genotype | ||
| IL-2-330 | 40.79 | 14.08 | 40.14 | 13.43 | > 0.05 |
| IFN-γ+874 | 11.55 | 35.02 | 14.65 | 38.10 | > 0.05 |
| IL-4-589 | 3.25 | 50.54 | 6.94 | 54.24 | > 0.05 |
| IL-10-592 | 42.60 | 10.83 | 43.40 | 11.64 | > 0.05 |
| IL-10-1082 | 1.08 | 35.02 | 10.91 | 44.85 | < 0.05 |
Table 4 Polymorphisms of IL-2-330, IFN-γ+874, IL-10-1082 and -592 and IL-4-589 and HBV and HCV infection n (%)
| Genotype allele | Controls (n = 74) | HCV infection (n = 55) | HBV infection (n = 69) | HBV/HCV coinfection (n = 79) | χ2 | P |
| IL-2-330 | ||||||
| TT | 22 (29.7) | 24 (43.6) | 33 (47.8) | 34 (43.0) | ||
| GG | 19 (25.7) | 6 (10.9) | 4 (5.8) | 10 (12.7) | 14.24 | 0.03 |
| TG | 33 (44.6) | 25 (45.5) | 32 (46.4) | 35 (44.3) | ||
| T | 77 (52.0) | 73 (66.4) | 98 (71.0) | 103 (65.2) | 12.33 | 0.01 |
| G | 71 (48.0) | 37 (33.6) | 40 (29.0) | 55 (34.8) | ||
| IFN-γ+874 | ||||||
| TT | 7 (9.5) | 5 (9.1) | 9 (13.0) | 11 (13.9) | ||
| AA | 14 (18.9) | 23 (41.8) | 25 (36.2) | 35 (44.3) | 16.15 | 0.01 |
| TA | 53 (71.6) | 27 (49.1) | 35 (50.7) | 33 (41.8) | ||
| T | 67 (45.3) | 37 (33.6) | 53 (38.4) | 55 (34.8) | 4.87 | 0.18 |
| A | 81 (54.7) | 73 (66.4) | 85 (61.6) | 103 (65.2) | ||
| IL-10-1082 | ||||||
| GG | 1 (1.4) | 2 (3.6) | 0 (0.0) | 0 (0.0) | ||
| AA | 16 (21.6) | 21 (38.2) | 27 (39.1) | 33 (41.8) | 13.05 | 0.04 |
| GA | 57 (77.0) | 32 (58.2) | 42 (60.9) | 46 (58.2) | ||
| G | 59 (39.9) | 36 (32.7) | 42 (30.4) | 46 (29.1) | 4.65 | 0.20 |
| A | 89 (60.1) | 74 (67.3) | 96 (69.6) | 112 (70.9) | ||
| IL-10-592 | ||||||
| AA | 34 (45.9) | 20 (36.4) | 29 (42.0) | 35 (44.3) | ||
| CC | 9 (12.2) | 6 (10.9) | 9 (13.0) | 6 (7.6) | 2.83 | 0.83 |
| AC | 31 (41.9) | 29 (52.7) | 31 (44.9) | 38 (48.1) | ||
| A | 99 (66.9) | 69 (62.7) | 89 (64.5) | 108 (68.4) | 1.10 | 0.78 |
| C | 49 (33.1) | 41 (37.3) | 49 (35.5) | 50 (31.6) | ||
| IL-4-589 | ||||||
| CC | 5 (6.8) | 1 (1.8) | 1 (1.4) | 2 (2.5) | ||
| TT | 38 (51.4) | 28 (50.9) | 35 (50.7) | 39 (49.4) | 4.41 | 0.62 |
| CT | 31 (41.9) | 26 (47.3) | 33 (47.8) | 38 (48.1) | ||
| C | 41 (27.7) | 28 (25.5) | 35 (25.4) | 42 (26.6) | 0.26 | 0.97 |
| T | 107 (72.3) | 82 (74.5) | 103 (74.6) | 116 (73.4) |
Table 5 Polymorphisms of IL-2-330, IFN-γ+874, IL-4-589, IL-10-1082 and -592 and clinical outcome n (%)
| Genotype | Control (n = 74) | Mild CH (n = 122) | Moderate/severe CH (n = 57) | Cirrhosis (n = 24) | χ2 | P |
| IL-2-330 | ||||||
| TT | 22 (29.7) | 54 (44.3) | 23 (40.4) | 13 (54.2) | ||
| GG | 19 (25.7) | 14 (11.4) | 6 (10.5) | 1 (4.2) | 13.46 | 0.04 |
| TG | 33 (44.6) | 54 (44.3) | 28 (49.1) | 10 (41.7) | ||
| T | 77 (52.0) | 163 (66.8) | 74 (64.9) | 36 (75.0) | 11.69 | 0.01 |
| G | 71 (48.0) | 81 (33.2) | 40 (35.0) | 12 (25.0) | ||
| IFN-γ+874 | ||||||
| TT | 8 (10.8) | 14 (11.5) | 5 (8.8) | 4 (16.7) | ||
| AA | 14 (18.9) | 48 (39.3) | 26 (45.6) | 9 (37.5) | 14.78 | 0.02 |
| TA | 52 (70.3) | 60 (49.2) | 26 (45.6) | 11 (45.8) | ||
| T | 68 (45.9) | 90 (36.9) | 36 (31.6) | 19 (39.6) | 5.68 | 0.13 |
| A | 80 (54.1) | 154 (63.1) | 78 (68.4) | 29 (60.4) | ||
| IL-10-1082 | ||||||
| GG | 1 (1.4) | 1 (0.8) | 1 (1.8) | 0 (0.0) | ||
| AA | 16 (21.6) | 44 (36.1) | 24 (42.1) | 13 (54.2) | 11.40 | 0.07 |
| GA | 57 (77.0) | 77 (63.1) | 32 (56.1) | 11 (45.8) | ||
| G | 59 (39.9) | 79 (32.4) | 34 (29.8) | 11 (22.9) | 5.85 | 0.12 |
| A | 89 (60.1) | 165 (67.6) | 80 (70.2) | 37 (77.1) | ||
| IL-10-592 | ||||||
| AA | 34 (45.9) | 51 (41.8) | 22 (38.6) | 11 (45.8) | ||
| CC | 9 (12.2) | 11 (9.0) | 8 (14.0) | 2 (8.3) | 2.50 | 0.87 |
| AC | 31 (41.9) | 60 (49.2) | 27 (47.4) | 11 (45.8) | ||
| A | 98 (66.2) | 163 (66.8) | 71 (62.3) | 33 (68.8) | 0.92 | 0.82 |
| C | 50 (33.8) | 81 (33.2) | 43 (37.7) | 15 (31.3) | ||
| IL-4-589 | ||||||
| CC | 3 (4.0) | 3 (2.4) | 3 (5.3) | 0 (0.0) | ||
| TT | 38 (51.4) | 69 (54.8) | 24 (42.1) | 11 (45.8) | 4.58 | 0.60 |
| CT | 33 (44.6) | 54 (42.9) | 30 (52.6) | 13 (54.2) | ||
| C | 39 (26.4) | 58 (23.8) | 36 (31.6) | 13 (27.1) | 2.46 | 0.48 |
| T | 109 (73.6) | 186 (76.2) | 78 (68.4) | 35 (72.9) |
Table 6 Polymorphisms of IL-2-330, IFN-γ+874, IL-4 -589 and IL-10-1082 and -592 and HCV RNA and ALT level n (%)
| HCV RNA - | HCV RNA + | χ2 | P | ALT < 80 U/L | ALT ≥ 80 U/L | χ2 | P | |
| IL-2-330 | ||||||||
| TT | 29 (37.2) | 39 (37.1) | 102 (41.8) | 11 (33.3) | ||||
| GG | 14 (17.9) | 14 (13.3) | 0.83 | 0.66 | 35 (14.3) | 4 (12.1) | 1.35 | 0.51 |
| TG | 35 (44.9) | 52 (49.5) | 107 (43.9) | 18 (54.5) | ||||
| T | 93 (59.6) | 130 (61.9) | 0.20 | 0.66 | 311 (63.7) | 40 (60.0) | 0.24 | 0.62 |
| G | 63 (40.4) | 80 (38.1) | 177 (36.3) | 26 (39.4) | ||||
| IFN-γ+874 | ||||||||
| TT | 13 (16.7) | 10 (9.5) | 29 (11.9) | 3 (9.1) | ||||
| AA | 28 (35.9) | 45 (42.9) | 2.36 | 0.31 | 85 (34.8) | 12 (36.4) | 0.23 | 0.89 |
| TA | 37 (47.4) | 50 (47.6) | 130 (53.3) | 18 (54.5) | ||||
| T | 63 (40.4) | 70 (33.3) | 1.92 | 0.17 | 188 (38.5) | 24 (36.4) | 0.12 | 0.74 |
| A | 93 (59.6) | 140 (66.7) | 300 (61.5) | 42 (63.6) | ||||
| IL-10-1082 | ||||||||
| GG | 1 (1.3) | 2 (1.9) | 3 (1.2) | 0 (0.0) | ||||
| AA | 14 (17.9) | 44 (41.9) | 12.27 | 0.00 | 81 (33.2) | 16 (48.5) | 3.25 | 0.20 |
| GA | 63 (80.8) | 59 (56.2) | 160 (65.6) | 17 (51.5) | ||||
| G | 91 (58.3) | 147 (70.0) | 5.36 | 0.02 | 166 (34.0) | 17 (25.8) | 1.79 | 0.18 |
| A | 65 (41.7) | 63 (128) | 322 (66.0) | 49 (74.2) | ||||
| IL-10-592 | ||||||||
| AA | 41 (52.6) | 44 (41.9) | 110 (45.1) | 8 (24.2) | ||||
| CC | 6 (7.7) | 11 (10.5) | 2.10 | 0.35 | 27 (11.1) | 3 (9.1) | 6.32 | 0.04 |
| AC | 31 (39.7) | 50 (47.6) | 107 (43.9) | 22 (66.7) | ||||
| A | 113 (72.4) | 138 (65.7) | 1.88 | 0.17 | 327 (67.0) | 38 (57.6) | 2.30 | 0.09 |
| C | 43 (27.6) | 72 (34.3) | 161 (33.0) | 28 (42.4) | ||||
| IL-4-589 | ||||||||
| CC | 3 (3.8) | 5 (4.8) | 6 (2.5) | 3 (9.1) | ||||
| TT | 48 (61.5) | 55 (52.4) | 1.53 | 0.47 | 132 (54.1) | 8 (24.2) | 12.46 | 0.02 |
| CT | 27 (34.6) | 45 (42.9) | 106 (43.4) | 22 (66.7) | ||||
| C | 33 (21.2) | 55 (26.2) | 1.24 | 0.27 | 118 (24.2) | 28 (42.4) | 9.97 | 0.00 |
| T | 123 (78.8) | 155 (73.8) | 370 (75.8) | 38 (57.6) |
- Citation: Gao QJ, Liu DW, Zhang SY, Jia M, Wang LM, Wu LH, Wang SY, Tong LX. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection. World J Gastroenterol 2009; 15(44): 5610-5619
- URL: https://www.wjgnet.com/1007-9327/full/v15/i44/5610.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.5610