Effects on coagulation factor production following primary hepatomitogen-induced direct hyperplasia

Table 1 Description of analyzed gene primers used in the real-time RT-PCR assay

Symbol	Gene name	Assay ID	Amplicon length (bp)
Housekeeping genes
PPIA	Peptidylprolyl isomerase A	Mm02342430_g1	148
RPL4	Ribosomal protein L4	Mm00834993_g1	129
Target genes
Fbg	Fibrinogen, beta chain	Mm00805336_ml	154
F2	Coagulation factor II (prothrombin)	Mm00438843_m1	68
F5	Coagulation factor V	Mm00484202_m1	61
F7	Coagulation factor VII	Mm00487329_m1	78
F8	Coagulation factor VIII	Mm00433174_m1	110
F9	Coagulation factor IX	Mm01308427_m1	74
F10	Coagulation factor X	Mm00484177_m1	81
F11	Coagulation factor XI	Mm00511167_m1	115
F12	Coagulation factor XII	Mm00491349_m1	64
F13b	Coagulation factor XIIIβ subunit	Mm00491938_m1	62
AT	Antithrombin	Mm00446573_m1	94
PC	Protein C	Mm00435966_m1	52
PS	Protein S (α)	Mm01343426_m1	62
Plg	Plasminogen	Mm00447087_m1	83
VWF	Von Willebrand factor	Mm00550376_m1	63
ADAMTS13	A disintegrin-like and metallopeptidase with thrombospondin type 1 motif, 13	Mm01218030_g1	77

Table 2 Increase in liver weight during the DH-mediated regenerative phase following TCPOBOP induction

	Body weight (g)	Liver weight (g)	LW/BW × 100
D0	22.44 ± 0.95	1.06 ± 0.06	4.73 ± 0.14
3 h	21.05 ± 0.88	1.10 ± 0.07	5.22 ± 0.26
D1	28.28 ± 2.08	1.63 ± 0.15	5.70 ± 0.16
D2	26.71 ± 1.49	2.05 ± 0.10	7.70 ± 0.12
D3	27.55 ± 2.29	2.38 ± 0.19	8.62 ± 0.09
D10	29.81 ± 0.91	3.15 ± 0.11	10.42 ± 0.16

LW/BW: Liver weight to body weight; TCPOBOP: 1,4-bis[2-(3,5-dichloropyridyloxy)] benzene. Quantitative changes in LW/BW ratio during the DH-regeneration phase at specific time points (D0, 3 h, D1, D2, D3, D10) following intragastric TCPOBOP injection (3 mg/kg body weight) (n = 8 mice/time point). Livers were harvested and weighed to calculate the relative liver weight (as a percentage of body weight). All values were represented as the mean ± SE.