Hepatoprotective evaluation of Anogeissus latifolia: In vitro and in vivo studies

Table 1 Effect of Anogeissus latifolia extract (ALE) on CCl₄-induced toxicity in rat hepatocytes (mean ± SE)

Treatment	Viable cells (%)	ALT (IU/L)	AST (IU/L)
Cell control	93.88 ± 0.81	18.70 ± 0.46	21.53 ± 0.63
Toxicant (1 mol/L CCl4)	33.06 ± 114	50.20 ± 0.61	57.50 ± 0.94
Std Liv52 (250 μL/mL)	85.84 ± 1.19 (86.7)a	19.46 ± 0.35 (97.59)a	24.10 ± 0.37 (92.85)a
ALE (250 μg/mL)	57.63 ± 1.58 (40.39)a	41.33 ± 0.62 (28.16)a	44.53 ± 1.25 (36.05)a
ALE (500 μg/mL)	77.60 ± 0.57 (73.72)a	38.50 ± 0.45 (37.14)a	32.80 ± 0.65 (68.66)a
ALE (1000 μg/mL)	81.24 ± 1.67 (79.20)a	22.23 ± 0.50 (88.48)a	27.33 ± 0.69 (83.87)a

Values in brackets indicate percentage protection against toxicant. Significant reduction compared to hepatotoxin (^aP < 0.05). ALT: Alanine aminotransferase; AST: Aspartate aminotransferase.

Table 2 Effect of Anogeissus latifolia on plasma biochemical parameters and lipid peroxidation in CCl₄-intoxicated rats liver (n = 6, mean ± SE)

Treatment (n = 6)	AST (IU/L)	ALT (IU/L)	ALP (IU/L)	Lipid peroxidation nmol of MDA/mg protein
Normal	88.17 ± 5.47	54 ± 2.7	249.5 ± 18.2	4.1 ± 0.5
CCl4 (500 μL/kg)	254.9 ± 19.3c	222.8 ± 10.14c	328.5 ± 25.36a	14.8 ± 1.3c
Liv52 (2 mL/kg)	111.7 ± 8.7b	71.4 ± 5.6b	255 ± 24.1b	5.4 ± 0.6b
ALE (300 mg/kg)	117 ± 6.7b	66.2 ± 6.1b	258 ± 15.54b	6.1 ± 0.5b

^aP < 0.01,

^cP < 0.001 vs normal;

^bP < 0.01 vs carbon tetrachloride. ALP: Alkaline phosphatase.