Overexpression of β3/γ2 chains of laminin-5 and MMP7 in biliary cancer

Table 1 Correlation between laminin γ2 staining and clinicopathologic factors n (%)

	Case (n = 61)	Laminin γ2		P value
		Positive (n = 35)	Negative (n = 26)
Age (yr)
≤ 65	25 (41)	15 (60)	10 (40)
> 65	36 (59)	20 (56)	16 (44)	0.47
Gender
Male	41 (67)	25 (61)	16 (39)
Female	20 (33)	10 (50)	10 (50)	0.29
Location
Bile duct	30 (49)	20 (67)	10 (33)
Gall bladder	18 (30)	9 (50)	9 (50)
Ampulla of Vater	13 (21)	6 (46)	7 (54)	0.34
Maximum diameter (mm)
< 30	41 (67)	25 (61)	16 (39)
≥ 30	20 (33)	10 (50)	10 (50)	0.29
Histologic type
Tub1 & pap	35 (57)	16 (46)	19 (54)
Others	26 (43)	19 (73)	7 (27)	0.03
Depth
Serosa negative	37 (61)	17 (46)	20 (54)
Serosa positive	24 (39)	18 (75)	6 (25)	0.02
Lymph node metastasis
Absent	29 (48)	16 (55)	13 (45)
Present	27 (44)	17 (63)	10 (37)	0.37
Stage
< IV	43 (70)	21 (49)	22 (51)
≥ IV	17 (28)	13 (76)	4 (24)	0.046

Table 2 Correlation between parameters and the pattern of laminin β3 expression n (%)

	Total (n = 61)	Laminin β3			P value
		Invasive (n = 23)	Diffuse (n = 17)	Negative (n = 21)
Age (yr)
≤ 65	25 (41)	9 (36)	8 (32)	8 (32)
> 65	36 (59)	14 (39)	9 (25)	13 (36)	0.83
Gender
Male	41 (67)	17 (41)	8 (20)	16 (39)
Female	20 (33)	6 (30)	9 (45)	5 (25)	0.11
Location
Bile duct	30 (49)	14 (47)	5 (17)	11 (37)
Gall bladder	18 (30)	5 (28)	7 (39)	6 (33)
Ampulla of Vater	13 (21)	4 (31)	5 (38)	4 (31)	0.40
Maximum diameter (mm)
< 30	40 (66)	18 (45)	10 (25)	12 (30)
≥ 30	21 (34)	5 (24)	7 (33)	9 (43)	0.27
Histologic type
Tub1 & pap	35 (57)	9 (26)	14 (40)	12 (34)
Others	26 (43)	14 (54)	3 (12)	9 (35)	0.02
Depth
Serosa negative	37 (61)	11 (30)	13 (35)	13 (35)
Serosa positive	24 (39)	12 (50)	4 (17)	8 (33)	0.18
Lymph node metastasis
Absent	29 (48)	8 (28)	11 (38)	10 (34)
Present	27 (44)	14 (52)	5 (19)	8 (30)	0.13
Stage
< IV	43 (70)	10 (23)	15 (35)	18 (42)
≥ IV	17 (28)	12 (71)	2 (12)	3 (18)	0.003

Table 3 Correlation between MMP7 staining and clinicopathologic factors n (%)

	Case (n = 61)	MMP7		P value
		Positive (n = 42)	Negative (n = 19)
Age (yr)
≤ 65	25 (42)	17 (68)	8 (32)
> 65	36 (58)	25 (69)	11 (31)	0.66
Gender
Male	41 (67)	30 (73)	11 (27)
Female	20 (33)	12 (60)	8 (40)	0.23
Location
Bile duct	30 (49)	24 (80)	6 (20)
Gall bladder	18 (30)	9 (50)	9 (50)
Ampulla of Vater	13 (21)	9 (69)	4 (31)	0.09
Maximum diameter (mm)
< 30	41 (67)	29 (71)	12 (29)
≥ 30	20 (33)	13 (65)	7 (35)	0.43
Histologic type
Tub1 & pap	35 (57)	23 (66)	12 (34)
Others	26 (43)	19 (73)	7 (27)	0.37
Depth
Serosa negative	37 (61)	25 (68)	12 (32)
Serosa positive	24 (39)	17 (71)	7 (29)	0.58
Lymph node metastasis
Absent	29 (48)	21 (72)	8 (28)
Present	27 (44)	18 (67)	9 (33)	0.43
Stage
< IV	43 (70)	31 (72)	12 (28)
≥ IV	17 (28)	10 (59)	7 (41)	0.24

Table 4 Expression of laminin γ2 and MMP7 in biliary tract carcinoma n (%)

MMP7 expression	Laminin γ2		P value
	Positive (n = 42)	Negative (n = 19)
Positive (n = 34)	28 (46)	6 (10)
Negative (n = 27)	14 (23)	13 (21)	0.01

Table 5 Expression of laminin β3 and MMP7 in biliary tract carcinoma n (%)

MMP7 expression	Laminin β3			P value
	Invasive (n = 23)	Diffuse (n = 17)	Negative (n = 21)
Positive (n = 34)	17 (28)	6 (10)	14 (23)
Negative (n = 27)	6 (10)	11 (18)	7 (11)	0.037

Table 6 Expression of laminin β3 and laminin γ2 in biliary tract carcinoma n (%)

Laminin γ2 expression	Laminin β3			P value
	Invasive (n = 23)	Diffuse (n = 17)	Negative (n = 21)
Positive (n = 42)	18 (30)	8 (13)	9 (15)
Negative (n = 19)	5 (8)	9 (15)	12 (20)	0.036