Checkpoint with forkhead-associated and ring finger promoter hypermethylation correlates with microsatellite instability in gastric cancer

Table 1 Summary of gastric cancer and methylation of CHFR

Case No.	Age (yr)	Sex	Lauren classification	CHFR methylation		MIN status2	P53 mutation
				Tumor	Normal
1	86	M	Intestinal	+	-	MSS	Mutant
2	67	F	Diffuse	+	-	MSI-L	Wild
3	76	F	Intestinal	+	+	MSS	Wild
4	30	F	Diffuse	-	-	MSS	Wild
5	74	M	Diffuse	-	+	ND1	Mutant
6	42	M	Diffuse	+	+	MSS	Wild
7	78	M	Diffuse	-	-	MSS	Wild
8	55	M	Diffuse	-	-	MSS	Mutant
9	50	F	Diffuse	-	-	MSS	Wild
10	55	M	Intestinal	-	-	MSS	Wild
11	63	M	Diffuse	-	-	MSS	Wild
12	69	M	Intestinal	-	+	MSS	Mutant
13	52	M	Intestinal	-	-	MSS	Wild
14	80	M	Diffuse	+	-	MSI-H	Wild
15	73	F	Diffuse	-	-	MSS	Wild
16	74	M	Intestinal	-	-	MSS	Wild
17	39	F	Diffuse	-	-	MSS	Wild
18	53	F	Diffuse	+	-	MSS	Wild
19	59	M	Diffuse	+	-	MSS	Mutant
20	72	M	Intestinal	+	-	MSS	Wild
21	69	M	Intestinal	-	-	MSS	Wild
22	51	M	Diffuse	-	-	MSS	Wild
23	61	M	Diffuse	-	-	MSI-L	Wild
24	65	M	Diffuse	-	-	MSI-L	Wild
25	56	M	Diffuse	-	-	MSS	Wild
26	53	M	Diffuse	-	-	MSS	Wild
27	42	M	Diffuse	-	-	MSI-H	Wild
28	59	F	Intestinal	-	-	MSS	Mutant
29	67	M	Intestinal	-	-	MSS	Mutant
30	55	F	Diffuse	-	-	MSS	Wild
31	68	M	Intestinal	+	-	MSI-L	Wild
32	73	M	Intestinal	-	-	MSS	Wild
33	49	F	Diffuse	+	-	MSS	Wild
34	52	F	Diffuse	-	-	MSS	Wild
35	69	M	Diffuse	-	-	MSS	Wild
36	73	M	Intestinal	+	-	MSS	Wild
37	63	M	Diffuse	-	-	MSS	Wild
38	73	F	Intestinal	-	-	MSS	Wild
39	65	M	Intestinal	-	-	MSS	Wild
40	60	M	Diffuse	-	-	MSS	Mutant
41	48	F	Diffuse	-	-	MSS	Wild
42	69	F	Diffuse	+	-	MSI-L	Wild
43	81	F	Intestinal	+	-	MSS	Wild
44	60	M	Intestinal	+	+	MSI-L	Wild
45	59	M	Intestinal	+	-	MSI-H	Wild
46	57	M	Diffuse	+	-	MSS	Wild
47	66	F	Intestinal	-	-	MSS	Wild
48	68	M	Intestinal	-	-	MSS	Wild
49	56	F	Intestinal	-	-	MSS	Wild
50	64	F	Diffuse	+	-	MSS	Wild
51	45	F	Diffuse	-	-	MSS	Wild
52	68	M	Diffuse	-	-	MSS	Wild
53	67	M	Diffuse	-	-	MSS	Wild
54	66	M	Diffuse	-	-	ND	Wild
55	55	M	Diffuse	+	-	MSI-H	Wild
56	52	F	Intestinal	+	-	MSS	Wild
57	77	F	Intestinal	+	-	MSS	Wild
58	61	M	Intestinal	-	+	ND	Wild
59	70	F	Diffuse	-	-	MSS	Wild

¹ND: Not detected;

²MSI-H: High level of miscrosatellite instability; MSI-L: Low level of microsatellite instability; MSS: Microsatellite stability.

Table 2 Clinicopathological features of gastric cancer and methylation of CHFR

Variable	Negative	Methylated	P value
	n= 39	n= 20
Gender
Male	26	11	NS
Female	13	9
Age	60.5	64.6	NS
Histology
Intestinal	14	10	NS
Diffuse	25	10
Serosal invasion
Negative	15	8	NS
Positive	24	12
Histological lymph node metastasis
Negative	15	5	NS
Positive	24	15
Vascular involvement
Negative	22	14	NS
Positive	14	6
Peritoneal dissemination
Negative	32	17	NS
Positive	7	3
Stage
I + II	12	5	NS
III + IV	27	15
p53 mutation
Wild	33	18	NS
Mutation	6	2
MIN status
MSI-H/L	3	7	< 0.01
MSS	36	13

NS: Not significant.