Relationship between angiotensin-(1-7) and angiotensin II correlates with hemodynamic changes in human liver cirrhosis

Table 1 Subject characteristics and casual measurements

Characteristics and measurements	Mild to moderate liver disease (MLD)	Advanced liver disease (ALD)	ALD during Liver transplantation (LT)
	n= 8	n= 7	n= 9
Age (yr)	55.5 ± 1.8	54 ± 5	50 ± 3
Sex, male/female	3 (37.5%)/5 (62.5%)	4 (57%)/3 (43%)	7 (78%)/2 (22%)
Child Pugh Score	6.7 ± 0.2	11 ± 0.8a	11 ± 1.8a
Albumin, g/dL	2.9 ± 0.15	2.4 ± 0.3	2.6 ± 0.2
Bilirubin, mg/dL	2.4 (1.7-5.9)	2.5 (1.2-7.1)	3.3 (2.0-5.5)
Creatinine, mg/dL	0.70 (0.70-0.80)	1.0 (1.0-1.45)a	1.0 (0.75-1.50)
INR (International normalization ratio)	1.2 ± 0.07	1.7 ± 0.3	1.8 ± 0.2
Prothrombin activity	68.3% ± 7.5%	50% ± 12%	42% ± 7%a
Serum Na+, mEq/L	139 ± 2	126 ± 3a	130 ± 2a

Data are expressed as mean ± SE or median (25 and 75 percentile), except for sex where number of patients and percentages are shown.

^aP < 0.05 for the comparison of the ALD and LT groups with the MLD group (ANOVA followed by Bonferroni test for mean comparisons and Kruskal-Wallis followed by the Dunn test for median comparisons). No statistical differences were detected between the ALD and LT groups.

Table 2 Circulating RAS components in healthy controls, MLD and ALD patients

RAS components	Healthy controls	MLD	ALD
PRA (ng Ang I/ mL per hour)	0.10 (0.03-0.23)	0.10 (0.01-0.22)	2.7 (0.43-6.61)ac
Ang I (pg/mL)	179.8 (86.9-220.8)	28.9 (23-65.2)a	412.3 (326.5-1123)ac
Ang II (pg/mL)	47.0 (41.8-61.7)	27.5 (23.9-35.9)a	84.4 (60.9-154.6)ac
Ang-(1-7) (pg/mL)	20.1 (17.1-25.5)	24.9 (21.1-27.4)	32.6 (27.8-61.6)a

Data are expressed as medians (25 and 75 percentile).

^aP < 0.05 for the comparison of MLD and ALD groups with healthy controls and

^cP < 0.05 for the comparison between MLD and ALD groups (Kruskal-Wallis followed by Dunn test). PRA: Plasma renin activity; Ang: Angiotensin.

Table 3 Peripheral and splanchnic RAS components during the pre-anhepatic stage of liver transplantation

RAS components	Peripheral measurements	Splanchnic measurements
PRA (ng Ang I/mL per hour)	2.4 ± 0.6	2.5 ± 0.4
Ang I (pg/mL)	764 ± 115	765 ± 103
Ang II (pg/mL)	138 ± 23	97 ± 13a
Ang-(1-7) (pg/mL)	48 ± 5	48 ± 7

Data are expressed as mean ± SE.

^aP < 0.05 for the comparison between peripheral and splanchnic measurements (paired Student t-test).