No association between cyclooxygenase-2 and uridine diphosphate glucuronosyltransferase 1A6 genetic polymorphisms and colon cancer risk

Table 1 Population characteristics n (%)

	Case (n = 422)	Control (n = 481)	P1
Age (mean ± SD) (yr)2	62.9 ± 10.6	57.9 ± 11.1	< 0.0001
Gender			0.0002
Female	203 (50.5)	304 (64.1)
Male	199 (49.5)	178 (35.9)
Race
Caucasian	378 (93.6)	449 (93.2)	0.35
African-American	21 (4.4)	21 (5.2)
Other	12 (2.5)	5 (1.2)
BMI (mean ± SD) (kg/m2)	29.2 ± 6.2	28.1 ± 6.1	< 0.0001
Family history3			0.0011
Yes	94 (24.0)	71 (15.2)
No	297 (76.0)	395 (84.8)
NSAID use			0.14
Regular	235 (64.2)	306 (69.1)
Irregular/none	131 (35.8)	137 (30.9)
Physical activity			0.008
None/low	111 (29.1)	113 (24.7)
Moderate	106 (27.8)	98 (21.4)
High	165 (43.2)	246 (53.8)
Regular alcohol use			0.04
Ever	134 (34.8)	191 (41.7)
Never	251 (65.2)	267 (58.3)
Smoking			0.88
Ever	207 (53.6)	248 (54.2)
Never	179 (46.4)	210 (45.9)

¹P-value of significance difference between cases and controls in χ² test (discrete variables and genotypes) or t-test (continuous);

²Age at diagnosis for cases, and age at questionnaire completion for controls;

³Family history of first-degree relatives with colorectal cancer.

Table 2 Odds ratios for individual SNPs and SNP by NSAID use interactions

SNP	Case/control	Crude		Adjusted		Stratified by NSAID use
		OR (95% CI)	P1	OR (95% CI)	P2	Regular	None	P3
COX2
rs2066826			0.45		0.34			0.41
GG	314/370	Ref		Ref		Ref	Ref
AG	98/99	1.17 (0.85, 1.60)		1.23 (0.88, 1.72)		1.05 (0.68, 2.64)	1.52 (0.82, 2.83)
AA	8/12	0.79 (0.32, 1.95)		1.06 (0.41, 2.73)		0.52 (0.10, 2.64)	1.96 (0.30, 12.62)
rs2206593			0.049		0.25			0.47
GG	382/417	Ref		Ref		Ref	Ref
AG	39/63	0.68 (0.44, 1.03)		0.69 (0.44, 1.07)		0.64 (0.36, 1.13)	0.89 (0.42, 1.90)
AA	0/2	-		-		-	-
rs5277			0.97		0.58			0.76
CC	310/353	Ref		Ref		Ref	Ref
CG	104/119	1.00 (0.73, 1.35)		0.90 (0.65, 1.25)		0.90 (0.59, 1.36)	0.86 (0.48, 1.54)
GG	7/8	1.00 (0.36, 2.78)		0.80 (0.27, 2.36)		0.52 (0.09, 2.95)	1.85 (0.32, 10.56)
rs689470			0.87		0.79			> 0.99
GG	380/440	Ref		Ref		Ref	Ref
AG	35/36	1.13 (0.69, 1.83)		1.15 (0.66, 2.02)		1.13 (0.54, 2.35)	1.59 (0.60, 4.22)
AA	4/4	1.16 (0.29, 4.66)		1.60 (0.26, 9.70)		-	2.38 (0.13, 43.43)
rs4648310			0.95		0.67			0.50
AA	405/462	Ref		Ref		Ref	Ref
AG	17/19	1.08 (0.55, 2.12)		1.07 (0.53, 2.19)		1.25 (0.48, 3.23)	0.69 (0.21, 2.27)
GG	0/1	-		-		-	-
rs5275			0.34		0.51			0.89
AA	176/216	Ref		Ref		Ref	Ref
AG	189/199	1.17 (0.88, 1.55)		1.17 (0.87, 1.58)		1.14 (0.78, 1.67)	1.04 (0.60, 1.79)
GG	56/65	1.06 (0.70, 1.59)		1.22 (0.79, 1.88)		0.98 (0.57, 1.71)	1.79 (0.78, 4.12)
rs689466			0.46		0.69			0.99
AA	275/297	Ref		Ref		Ref	Ref
AG	138/168	0.89 (0.67, 1.17)		0.86 (0.64, 1.15)		0.91 (0.62, 1.33)	0.83 (0.48, 1.42)
GG	9/15	0.65 (0.28, 1.51)		0.65 (0.26, 1.60)		0.75 (0.26, 2.17)	0.69 (0.11, 4.45)
rs20417			0.41		0.20			0.44
GG	291/343	Ref		Ref		Ref	Ref
CG	119/121	1.16 (0.86, 1.56)		1.25 (0.91, 1.71)		1.17 (0.78, 1.74)	1.39 (0.78, 2.47)
CC	11/15	0.86 (0.39, 1.91)		0.97 (0.43, 2.20)		0.46 (0.14, 1.54)	3.12 (0.57, 17.15)
rs2745557			0.61		0.41			0.18
GG	287/321	Ref		Ref		Ref	Ref
AG	120/141	0.95 (0.71, 1.27)		0.93 (0.69, 1.27)		1.04 (0.70, 1.54)	0.77 (0.43, 1.38)
AA	13/19	0.76 (0.37, 1.58)		1.01 (0.48, 2.14)		1.49 (0.60, 3.67)	0.34 (0.07, 1.68)
UGT1A6
rs1105879			0.46		0.28			0.93
AA	191/206	Ref		Ref		Ref	Ref
AC	167/209	0.86 (0.65, 1.14)		0.84 (0.63, 1.14)		0.84 (0.57, 1.23)	0.86 (0.50, 1.48)
CC	64/66	1.05 (0.70, 1.55)		1.07 (0.71, 1.64)		1.20 (0.70, 2.06)	1.07 (0.50, 2.32)
rs2070959			0.21		0.21			0.93
AA	208/207	Ref		Ref		Ref	Ref
AG	154/206	0.78 (0.59, 1.03)		0.78 (0.58, 1.04)		0.76 (0.52, 1.12)	0.82 (0.48, 1.42)
GG	59/57	1.08 (0.72, 1.63)		1.11 (0.72, 1.72)		1.26 (0.72, 2.23)	1.04 (0.47, 2.29)

¹P-value of SNP in best fitting genetic model in the unadjusted logistic regression; ²P-value of SNP in best fitting genetic model in the full multivariate logistic regression; ³P-value of interaction of SNP in best fitting genetic model with regular NSAID use in the logistic regression; ^aP < 0.05.