Intestinal microflora molecular markers of spleen-deficient rats and evaluation of traditional Chinese drugs

Table 1 Clinical recipes of traditional Chinese drugs used in this study

Prescription	Composition	Dose (g crude plants/kg)	Effecta
Banxia Houpu Tang	Rhizoma Pinelliae, Poria, Cortex Magnoliae Officinalis, Folium Perillae, Rhizoma Zingiberis Recens (4:4:3:3:2)	4.3 (clinical dose)	N↓, A↓, C↓, H↓
Si Junzi Tang	Radix Ginseng, Rhizoma Atractylodis Macrocephalae, Poria, Radix Glycyrrhizae (10:9:9:6)	1.2 (triplicate of clinical dose)	N↓1, A↓1, C↑, H↑
		3.5 (clinical dose)	N↓1, A↓1, C↑1, H↑
		10.5 (triplication of clinical dose)	N↓1, A↓, C↑, H↓
Lizhong Tang	Radix Codonopsis, Rhizoma Atractylodis Macrocephalae, Rhizoma Zingiberis, Radix Glycyrrhiza preparata (1:1:1:1)	1.8 (triplicate of clinical dose)	N↓, A↓, C↑, H↑
		5.4 (clinical dose)	N↓1, A↓1, C↑, H↑
		16.2 (triplication of clinical dose)	N↓1, A↓, C↑, H↑
Buzhong Yiqi Tang	Radix Astragali, Radix Ginseng, Radix Angelicae Sinensis, Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhiza preparata, Radix Bupleuri, Rhizoma Cimicifugae, Pericarpium Citri Reticulatae (6:1:1:1:3:2:2:2)	1.6 (triplicate of clinical dose)	N↓1, A↓1, C↑, H↑
		4.8 (clinical dose)	N↓1, A↓1, C↑1, H↑
		14.4 (triplication of clinical dose)	N↓1, A↓, C↑, H↓
Yiwei Tang	Radix Glehniae, Raidix Ophiopogonis, Rehmannia Dride Rhizome, Rhizoma Polygonati Odorati, rock candy (3:3:3:1:3)	1.9 (triplicate of clinical dose)	N↓1, A↓1, C↑, H↓
		5.8 (clinical dose)	N↓1, A↓1, C↑, H↓
		17.4 (triplication of clinical dose)	N↓, A↓, C↑, H↓

^aN: Net area of 380 bp; A: Abundance of 380 bp; C: Sorenson’s pairwise similarity coefficient (Cs); H: Shannon’s index (H'); ↑: Increase; ↓: Decrease;

¹: Significant (P < 0.05).

Table 2 Occurrence and preliminary biomarkers of intestinal microflora ERIC-PCR fingerprinting in rats with Pi-deficiency induced by Radix and Rhizoma Rhei (n = 100)

Fragments	Occurrence (%)		Net integral area			Abundance
	Beforeadministration(healthy)	Afteradministration(Pi-deficiency)	Beforeadministration(healthy)	Afteradministration(Pi-deficiency)	Range	Before administration (healthy)	Afteradministration(Pi-deficiency)	Range
590 bp	79	53	851.65 ± 68.00	385.76 ± 37.63a	Decrease 55%	18.49 ± 1.36	11.69 ± 1.05a	Decrease 37%
380 bp	33	94	98.61 ± 18.09	563.64 ± 32.94a	Increase 470%	2.74 ± 0.58	19.16 ± 1.30a	Increase 590%
300 bp	75	10	604.93 ± 46.67	70.87 ± 23.12a	Decrease 88%	12.70 ± 0.94	2.31 ± 0.71a	Decrease 82%

Paired samples t test,

^aP < 0.05 vs before administration.