Bile-acid-activated farnesoid X receptor regulates hydrogen sulfide production and hepatic microcirculation

Table 1 Primers used for quantitative and qualitative PCR

Gene	Forward	Reverse
hGAPDH	GAAGGTGAAGGTCGGAGT	CATGGGTGGAATCATATTGGAA
hCSE	CACTGTCCACCACGTTCAAG	GTGGCTGCTAAACCTGAAGC
hCSE-IR1	CATTACAGAGTTCAGTGTACCT	GCAGCTTGGATTCTCATCAGTC
r18S	GCAATTATTCCCCATGAACG	GGCCTCACTAAACCATCCAA
rCSE	GTATTGAGGCACCAACAGGT	GTTGGGTTTGTGGGTGTTTC
rFXR	TGGACTCATACAGCAAACAGAGA	GTCTGAAACCCTGGAAGTCTTTT
rαSMA	GCTCCATCCTGGCTTCTCTA	TAGAAGCATTTGCGGTGGAC
rCOL1α1	TGCTGCCTTTTCTGTTCCTT	GGATTTGAAGGTGCTGGGTA
rSHP	CCTGGAGCAGCCCTCGTCTCAG	AACACTGTATGCAAACCGAGGA
m18S	ACCGCAGCTAGGAATAATGGA	GCCTCAGTTCCGAAAACCA
mCSE	TGCTGCCACCATTACGATTA	GATGCCACCCTCCTGAAGTA
mα1-collagen	ACGTCCTGGTGAAGTTGGTC	CAGGGAAGCCTCTTTCTCCT

h: Human; m: Mouse; r: Rat; hCSE-IR1: Primers used for real-time PCR of the CSE promoter in chromatin immunoprecipitation assay.

Table 2 Effect of loss of FXR on liver injury induced by 12 administrations of CCl₄ (6 wk)

	AST (U/L)	ALT (U/L)	Bilirubin (mg/dL)
FXR +/+ naive	127 ± 15	48 ± 8	0.015 ± 0.001
FXR +/+ CCl4	250 ± 40a	369 ± 35a	0.118 ± 0.004a
FXR -/- naive	143 ± 10	79 ± 25a	0.068 ± 0.004a
FXR -/- CCl4	740 ± 230ce	354 ± 137c	0.252 ± 0.02ce

Data are mean ± SD of six mice.

^aP < 0.05 vs FXR +/+ control mice;

^cP < 0.05 vs FXR -/- control mice;

^eP < 0.05 vs CCl₄ FXR +/+ mice.