Therapeutic effects of Clostridium butyricum on experimental colitis induced by oxazolone in rats

Table 1 Effects on body weight and wet colon mass index in rat colitis induced with oxazolone (mean ± SD)

Group	n	Dose	Body weight (g)	Wet colon mass index (g/kg)
NC	8	N/A	191.3 ± 24.2	5.3 ± 1.2
PC	7	N/A	149.0 ± 15.9d	9.1 ± 2.5d
PB	10	2.3 × 1011 CFU/L	175.9 ± 43.2	6.7 ± 1.3a
MA	8	100 g/L	179.8 ± 18.4	6.9 ± 2.3
SB	8	0.05 mol/L	175.8 ± 23.0	6.5 ± 1.6a

^aP < 0.05 vs positive control;

^dP < 0.01 vs negative control; NC: Negative control; PC: Positive control; PB: Probiotic (C. butyricum); MA: Mesalamine; SB: Sodium butyrate.

Table 2 IL-23 and TNF-α levels in rat serum in oxazolone-induced experimental colitis

Group (n = 7)	Dose	IL-23 (ng/L)	TNF-α(ng/L)
NC	N/A	5.75 ± 2.51	15.93 ± 11.36
PC	N/A	43.94 ± 20.36d	28.17 ± 6.10c
PB	2.3 × 1011 CFU/L	5.99 ± 1.88b	16.05 ± 10.54a
MA	100 g/L	8.81 ± 3.78b	23.54 ± 11.03
SB	0.05 mol/L	8.38 ± 4.48b	23.18 ± 6.48

^aP < 0.05,

^bP < 0.01 vs PC;

^cP < 0.05,

^dP < 0.01 vs NC. PC: Positive control; NC: Negative control; PB: Probiotic (C. butyricum); MA: Mesalamine; SB: Sodium butyrate.

Table 3 Effect on rat intestinal bacterial balance of oxazolone-induced experimental colitis (n = 6, log10ⁿ CFU/g)

Group	Colibacter	Bifidobacterium	Acidobacterium	Fusobacterium	Clostridium
NC	6.87±0.4	9.42 ± 0.25	9.61 ± 0.12	2.92 ± 0.42	5.10 ± 0.19
PC	7.54 ± 0.13d	8.88 ± 0.17d	9.01 ± 0.15d	4.37 ± 0.09d	5.69 ± 0.11d
PB	6.56 ± 0.35b	9.62 ± 0.24b	9.92 ± 0.05b	3.57 ± 0.23b	4.85 ± 0.17b
MA	7.16 ± 0.20af	9.54 ± 0.16b	9.48 ± 0.13bf	3.79 ± 0.02b	4.39 ± 0.29b
SB	6.72 ± 0.18be	9.46 ± 0.59b	9.51 ± 0.09bf	3.72 ± 0.02b	4.79 ± 0.18be

^aP < 0.05,

^bP < 0.01 vs PC;

^dP < 0.01 vs NC;

^eP < 0.05,

^fP < 0.01 vs PB. NC: Negative control; PC: Positive control; PB: Probiotic (C. butyricum); MA: Mesalamine; SB: Sodium butyrate.