Inhibitory effect of acetylshikonin on human gastric carcinoma cell line SGC-7901 in vitro and in vivo

Table 1 Sequence of primers used in our study

	Product size	Sense primer	Anti-sense primer
Bax	311 bp	5′GCGTCCACCCAAGAAGCTGAG3′	5′ACCACCCTGGTCTTGGATCC3′
Bcl-2	280 bp	5′TGTGGCCTTCTTTGAGTTCG3′	5′TCACTTGTGGCTCAGATAGG3′
β-actin	300 bp	5′TCACCCACACTGTGCCCATCTACGA3′	5′CAGCGGAACCGCTCATTGCCAATGG3′

Table 2 Effect of acetylshikonin on cell-cycle phase distribution and apoptotic ratios of SGC-7901 cells in vitro (mean ± SD)

Concentration (mg/L)	Cell cycle phase distribution (%)			Apoptotic index (%)
	G0/G1	S	G2/M	Hoechst	TUNEL	FACScan
0	53.73 ± 4.61	36.29 ± 7.32	9.98 ± 2.35	5.76 ± 0.58	5.34 ± 0.64	6.15 ± 0.75
0.2	54.82 ± 4.12	33.45 ± 6.05	11.73 ± 3.90	11.05 ± 2.10a	11.56 ± 1.62a	10.13 ± 1.69a
0.4	56.92 ± 5.04	30.15 ± 5.84	12.93 ± 5.32	28.76 ± 3.55b	30.89 ± 2.35b	25.40 ± 2.46b
0.8	57.12 ± 2.94	28.58 ± 5.21	14.30 ± 4.79a	52.25 ± 6.16b	56.33 ± 5.78b	48.73 ± 6.55b

Different concentrations of acetylshikonin were added to SGC-7901 cell culture for 24 h, cell-cycle phase distribution and apoptotic ratios were then analyzed by FACScan flow cytometry and TUNEL assay with Hoechst staining. Each experiment was independently performed three times.

^aP < 0.05,

^bP < 0.01 vs control group.