Hirschsprung’s disease: Is there a relationship between mast cells and nerve fibers?

Table 1 Mast cells in Hirschsprung’s disease (HD) and non-HD cases

	HD cases				Non-HD cases
S. No.	Age (mo)	Sex	No. of mast cells	S. No.	Age (mo)	Sex	No. of mast cells
1	36	M	35	1	30	F	1
2	36	M	40	2	24	M	15
3	12	F	35	3	36	M	25
4	24	M	35	4	36	F	5
5	3	M	10	5	4	M	10
6	36	M	30	6	48	M	3
7	5	M	50	7	1	M	1
8	5	M	20	8	1	M	25
9	36	F	70	9	96	M	15
10	24	F	20	10	1	F	32
11	84	M	45	11	2	M	15
12	36	M	45	12	30	F	25
13	84	M	80	13	36	M	15
14	18	F	30	14	48	M	20
15	36	M	45
16	48	M	26
17	48	M	13
18	12	F	27

HD (mean ± 2 SD = 36.44 ± 36.02) and non-HD (mean ± 2 SD = 14.79 ± 19.8) [(HD vs non-HD) P = 0.0001].

Table 2 Comparison of mast cells in submucosa of HD and non-HD cases with previous studies

Mast cells per 5 hpf in submucosa		Kobayashi et al[5]	Demirbilek et al[6]	Present study
HD cases	Aganglionic segment	23.9 ± 6.6	18.2 ± 3.3	36.36 ± 39.58
	Ganglionic segment	8.5 ± 3.9	1.7 ± 0.4	38 ± 16.66
Non-HD cases		7.2 ± 3.4	5.4 ± 1.2	14.8 ± 5.3

Aganglionic segment (Kobayashi et al vs Present study, P = 0.0001; Demirbilek et al vs Present study, P = 0.0001); Ganglionic segment (Kobayashi et al vs Present study, P = 0.0001; Demirbilek et al vs Present study, P = 0.0001); Non HD cases (Kobayashi et al vs Present study, P = 0.0001; Demirbilek et al vs Present study, P = 0.0001).

Table 3 Number, size and perimeter of nerve fibers in HD and non-HD cases

	HD cases					Non-HD cases
S. No.	Age (mo)	Sex	No. of nerve fibers	Size of nerve fibers (&mgr;m)	Perimeter of nerve fibers (&mgr;m)	Age (mo)	Sex	No. of nerve fibers	Size of nerve fibers (&mgr;m)	Perimeter of nerve fibers (&mgr;m)
1	42	M	13	40.37	230.48	36	M	3	42.5	215.7
2	12	F	8	68.0	195.9	1	M	2	11.2	37.0
3	36	M	11	65.4	172.8	5	M	4	15.3	47.3
4	84	M	7	47.3	135.4	1	M	4	15.0	51.8
5	48	M	16	63.5	288.75	1	F	3	40.6	135.56
6	36	M	10	77.38	237.79	30	F	3	25.0	126.67
7	48	M	12	57.5	256.11	4	M	9	28.8	83.2
8	36	M	14	40.7	149.3	13	M	13	69.9	208.09
9	12	M	6	55.5	190.03
10	24	M	11	47.6	184.08
11	1	M	15	49.6	178.18
12	60	M	11	73.4	203.9

Number of nerve fibers (HD vs non-HD), P = 0.0001; Size of nerve fibers (HD vs non-HD), P = 0.0001; Perimeter of nerve fibers (HD vs non-HD), P = 0.0001.