Isolation and bioinformatics analysis of differentially methylated genomic fragments in human gastric cancer

Table 1 Adaptors and sequences in MS-RDA

Primer	Sequences
RHpaII24	5’-AGC ACT CTC CAG CCT CTC ACC GAC-3’
RHpaII11	5’-CGG TCG GTG AG-3’
JHpaII24	5’-ACC GAC GTC GAC TAT CCA TGA AAC-3’
JHpaII11	5’-CGG TTT CAT GG-3’
NHpaII24	5’-AGG CAA CTG TGC TAT CCG AGG GAC-3’
NHpaII11	5’-CGG TCC CTC GG-3’
SHpaII24	5'-ACT TCT ACG GCT GAA TTC CGC CAC-3'
SHpaII11	5’-CGG TGT CGG AAT-3’

MS-RDA: Methylation-sensitive representational difference analysis.

Table 2 Characteristics of differentially methylated DNA fragments by MS-RDA

Accession number	Screening time	Size (bp)	GC (%)	CG/GC	Similarity
					Location	S	E Similarity rate (%)
AY887106	2	395	50.6	0.9286	8q13.3	745	0	98
AY887107	2	264	66.3	0.7000	2p11.1	521	e-145	99
					1q21.1	498	e-138	98
					4p16.1	435	e-119	94