Persistence of side population cells with high drug efflux capacity in pancreatic cancer

Table 1 Sequences of human primers used for real-time PCR experiments

Target mRNA	Primer sequence (5’-3’)	Accession number	Size (bp)
ABCB1	5′AGGTTCCAGGATTGGCGTCTT3′	AF399931	156
	5′CCAGTCATTGCTGCGGTTTCA3′		1152-1307
ABCG2	5′AATACATCAGCGGATACTACAGAG3′	AY333756	179
	5′AGCCACCATCATAAGGGTAAACAT3′		1382-1560
β-actin	5’GAACGGTGAAGGTGACAG3’	NM_001101	293
	5’TAGAGAGAGTGGGGTGG3’		915-1207

Table 2 Relative quantitation of mRNA using the 2^-ΔΔCT method

	Target mRNA	ΔCT	Target mRNA
		(Target mRNA-β-actin)	related toβ-actin
non-SP cells	ABCB1	5.82 ± 1.16	0.0176
			(0.0079-0.0393)
	ABCG2	5.48 ± 0.94	0.0224
			(0.0116-0.0432)
SP cells	ABCB1	1.15 ± 0.72	0.4504
			(0.2731-0.7431)
	ABCG2	1.16 ± 0.75	0.4483
			(0.2662-0.7547)